INTEGRA Biosciences has collaborated closely with nucleic acid extraction experts MACHEREY-NAGEL (MN) to develop automated protocols for a variety of NucleoSpin RNA and DNA extraction and purification kits on the ASSIST PLUS pipetting robot.   The combination of these technologies provides high quality, purity and yield for DNA and RNA extracts from varied specimen types, suitable for use in a range of applications.

Each MN kit comes in a 96 well format, and can be easily automated on the ASSIST PLUS using a 12 channel 1250 μl VIAFLO electronic pipette   with 1250 μl Sterile, Filter GripTips. Preset, biologically validated protocols can be quickly downloaded through VIALAB software, offering walk-away pipetting and incubation for:

• MN NucleoSpin 96 RNA Extraction kit – for extracting and purifying RNA fragments (>200 nt) from cell cultures, tissues and saliva in high throughput.
• MN NucleoSpin 96 Tissue kit – for high throughput extraction and purification of high molecular weight genomic DNA from animal tissue samples or animal/bacterial cells.
• MN NucleoSpin 96 Plasmid kit – for parallel extraction and purification of high-copy plasmid DNA with a vector size of less than 25 kbp.
• MN NucleoSpin 96 Plasmid Transfection-grade kit – for high throughput extraction and purification of high-copy, transfection-grade E. coli plasmid DNA with a vector size <25 kbp.

Automating liquid handling tasks using the ASSIST PLUS eliminates ergonomic hazards and provides more hands-free time for the user. Pipetting errors and variability between users are avoided, and the validated workflows consistently guarantee maximum reproducibility. In addition, the flexibility of this system ensures that it will always have a purpose in the lab, and can be used to simplify a wide range of general life sciences and diagnostics tasks for far-reaching applications.

• For more information, visit: INTEGRA Biosciences

 

Torrey Pines Scientific, has introduces its new EchoTherm HPLC Column Oven Model CO20. The temperature range is from room temperature to 90.0°C readable and settable to 0.1°C. The PID temperature control software regulates temperatures to ±0.1°C. Temperature accuracy and stability are ±0.1ºC with a stable temperature indicator lamp on the front panel that lights when the target temperature is stable to within ±0.2ºC.

The unit holds columns up to 30 cm long by ¼” or 3/8” diameter in mounting clips provided with room for guard columns and fittings. Larger diameter columns may be used by removing the column clips that hold the smaller columns.

The CO20 features simple controls, digital display of target and actual chamber temperatures, an injection counter, and 30-day timer with alarm and user settable Auto-Off. An accessory stand is available.

The unit operates from 12 volts DC and comes with a bench top universal power supply for use anywhere in the world, 3-wire AC line cord for the country of use, counter cable, twelve month warranty and instruction manual. The Model CO20 is UL, CSA, and CE compliant.

• For more information, visit: torreypinesscientific.com.

London-based PCR Biosystems has launched Air-Dryable Probe 1-Step Mix, a versatile all-in-one, air-dryable 4x RT-qPCR reagent mix for ultra-sensitive detection of RNA and DNA sequences. With this kit, the robust performance of the company’s liquid PCR reagents can be brought to dried assay formats using a standard laboratory oven, offering a simpler, faster, and cheaper alternative to lyophilisation (freeze-drying).

Now, a larger base of assay kit developers, including smaller organisations with fewer resources, can access the advantages of dried assays — shorter time-to-market, simplified logistics and storage, and significantly reduced costs. Sectors set to realise such benefits include clinical, veterinary, food, environmental and agricultural testing.

Assay developers moving toward dried test formats

The costly cold-chain shipping and storage demands of traditional ‘wet’ RT-qPCR reagents and tests are increasingly driving assay developers toward dried test formats. But while lyophilised assays have emerged as a powerful option that can reduce test weight, volume, and transport and storage requirements, they can be out of reach for smaller, resource-strapped organisations. This is because lyophilisation requires expensive, specialised equipment, deep technical expertise, and extensive drying times. Outsourcing lyophilisation alleviates some of this burden, but it entails loss of process control.

With the new air-dryable kits, drying can be done in just 80 minutes with a simple laboratory oven, without extensive training, and at a much lower cost. As a result, organisations unable to build onsite lyophilisation capabilities can now create high-performance dried assays in-house, with full control over the drying process and quality control steps. The new kits also do not suffer from the restrictive drying protocol rigidity that can often complicate lyophilisation. Further, air-dried assays are less susceptible to moisture and so do not require the strict humidity control during manufacturing that is needed with lyophilised tests. This is particularly useful in high-humidity regions and allows local diagnostic kit developers to more confidently expand their portfolio into dried formats.

As with the company’s lyophilisable mixes, the air-dryable mixes retain performance after reconstitution. Kits also come with the company’s renowned technical support to ensure smooth troubleshooting and optimal results, and free samples are available.

“Dryable reagents are redefining the ease and convenience with which PCR assays are being developed, distributed, and used. Lyophilisation has played a huge part here, but barriers to its adoption have seen smaller companies get left behind,” said Alex Wilson, co-founder of PCR Biosystems. “Our air-dryable mixes erode barriers to drying, so even with minimal resources, developers can get superior-quality dried assays to the point of care, efficiently. This is the latest step in our journey to place simple, cost-effective, and high-performing tests within reach of all.”

What’s in the Air-Dryable Probe 1-Step Mix kit?

The new kits comprise a glycerol-free 4x qPCR mix with hot start Taq polymerase, dNTPs, MgCl₂ and a blend of excipients for reliable drying without activity loss. A more stable version of UltraScript Reverse Transcriptase and RiboShield RNase inhibitor are blended into the mix, providing a more convenient single-tube format. Kits can be used for both RNA and DNA detection for greater versatility, and dried assays are stable for 3 months at 37°C.

Critically, PCR Biosystems is certified to conform to Quality Management System standard ISO 13485, ensuring full product traceability, and optimum lot-to-lot consistency. Assay developers can therefore be confident in using the new air-dryable kits to create the highest-quality tests.

• For more information, visit: Air-Dryable Probe 1-Step Mix
• Free samples can be requested via this form.

 

Porvair Sciences has developed a 2 ml 96-well microplate designed for labs processing cannabis samples for analysis of Tetrahydrocannabinol (THC) and Cannabidiol (CBD).

Sample processing is the second step in testing cannabis strains. This is carried out after collecting leaf, bud, and flower samples from cannabis plants, ready for accurate determination of the THC/CBD ratio by HPLC.

Traditionally sample processing protocols have used bead-beating or grinding mills to homogenize the plant materials. When this sample processing is carried out in standard polypropylene microplates, the extreme forces applied by these machines can cause damage to the plates, leading to cracking, leaking, and incorrect results due to sample cross-contamination.

To address these design shortfalls, Porvair Sciences developed a reinforced microplate that could withstand the applied forces in bead beaters while maintaining a standard ANSI/SLAS footprint making the methodology automation compatible.

Based upon a 2 ml square well block design, Porvair Sciences cannabis sample processing microplate sets a new standard for reliable, high productivity cannabis plant processing. To ensure that no contamination from the plate will affect the THC/CBD analysis, Porvair Sciences only uses pure, extractables-free polypropylene, to manufacture its plates.

• For more information, visit; Porvair’s 96-well microplate designed for labs processing cannabis samples