{"id":14435,"date":"2021-05-24T10:46:34","date_gmt":"2021-05-24T10:46:34","guid":{"rendered":"https:\/\/clinlabint.com\/?p=14435"},"modified":"2021-06-01T12:44:50","modified_gmt":"2021-06-01T12:44:50","slug":"evaluation-of-recovery-of-basic-analytes-with-microlute-cp-30-mg-mixed-mode-strong-cation-exchange-scx-spe-by-hplc-ms","status":"publish","type":"post","link":"https:\/\/clinlabint.com\/evaluation-of-recovery-of-basic-analytes-with-microlute-cp-30-mg-mixed-mode-strong-cation-exchange-scx-spe-by-hplc-ms\/","title":{"rendered":"Evaluation of Recovery of Basic Analytes with Microlute\u00ae CP 30 mg Mixed-Mode Strong Cation Exchange (SCX) SPE by HPLC-MS"},"content":{"rendered":"
Sample preparation is important in any type of chromatography analysis. While it can add on extra time, the process of cleaning up samples before injection onto a system results in a range of benefits to the analyst \u2013 better recoveries, more reproducible analysis, less downtime of instruments, reduction of troubleshooting, as well as less complex chromatograms due to the reduction of unwanted compounds being injected. All of these can result in time saved which could be needed for repeated work or maintenance on instruments.<\/p>\n
Traditional SPE products consist of a loose-filled resin sandwiched between two frits. While this is known to work, it can come with some problems which can complicate analysis or result in poor data being produced. These problems are a result from how the product is packed into a well or cartridge \u2013 voiding can occur under the top frit, channels could form through the resin bed which can cause less efficient interactions between the resin and the analyte(s) or there could be variation on compression or resin weight that was dosed into each product.<\/p>\n