{"id":14435,"date":"2021-05-24T10:46:34","date_gmt":"2021-05-24T10:46:34","guid":{"rendered":"https:\/\/clinlabint.com\/?p=14435"},"modified":"2024-06-06T11:39:15","modified_gmt":"2024-06-06T11:39:15","slug":"evaluation-of-recovery-of-basic-analytes-with-microlute-cp-30-mg-mixed-mode-strong-cation-exchange-scx-spe-by-hplc-ms","status":"publish","type":"post","link":"https:\/\/clinlabint.com\/evaluation-of-recovery-of-basic-analytes-with-microlute-cp-30-mg-mixed-mode-strong-cation-exchange-scx-spe-by-hplc-ms\/","title":{"rendered":"Evaluation of Recovery of Basic Analytes with Microlute\u00ae CP 30 mg Mixed-Mode Strong Cation Exchange (SCX) SPE by HPLC-MS"},"content":{"rendered":"

Background<\/span><\/h2>\n

Sample preparation is important in any type of chromatography analysis. While it can add on extra time, the process of cleaning up samples before injection onto a system results in a range of benefits to the analyst \u2013 better recoveries, more reproducible analysis, less downtime of instruments, reduction of troubleshooting, as well as less complex chromatograms due to the reduction of unwanted compounds being injected. All of these can result in time saved which could be needed for repeated work or maintenance on instruments.<\/p>\n

Traditional SPE products consist of a loose-filled resin sandwiched between two frits. While this is known to work, it can come with some problems which can complicate analysis or result in poor data being produced. These problems are a result from how the product is packed into a well or cartridge \u2013 voiding can occur under the top frit, channels could form through the resin bed which can cause less efficient interactions between the resin and the analyte(s) or there could be variation on compression or resin weight that was dosed into each product.<\/p>\n

\"Common

Figure 1. Common issues associated with loose-filled SPE methods<\/p><\/div>\n

The Microlute\u00ae CP SPE products consist of a unique hybrid design of a solid interconnected network of evenly distributed pores combined with retentive media. The advantage of this design is that flow through the product is consistent and increases the interaction between the analytes and retentive media present within the structure. These two features combined results in a product which offers both high recovery values as well as reproducible results. This technical note uses the 30 mg Microlute\u00ae CP Strong Cation Exchange (SCX) 96 well plate to compare performance in recoveries and reproducibility against five competitor loose-filled 30 mg SCX products.<\/p>\n

\"Schematic

Figure 2. Schematic of the hybrid polymeric structure showing the porous structure of the frit with the active resin immobilised throughout the pore structure<\/p><\/div>\n

Introduction<\/span><\/h2>\n

The Microlute\u00ae CP SCX product is a mixed-mode polymeric SPE product – a combination of ion exchange and reversed phase. This results in a product that has two retention mechanisms which can be fine-tuned to allow more flexibility in the SPE method. The reversed phase functionality allows for separation of analytes on hydrophobic interactions, allowing for retention to be altered by organic modifier concentration. Whereas ion exchange allows for selective strong ionic interactions between the resin and the charged analytes. The introduction of polymeric resins to SPE has resulted in some extra advantages over using silica-based resins [1]. These include:<\/p>\n