{"id":15460,"date":"2021-09-13T07:05:43","date_gmt":"2021-09-13T07:05:43","guid":{"rendered":"https:\/\/clinlabint.com\/?p=15460"},"modified":"2024-06-06T11:45:13","modified_gmt":"2024-06-06T11:45:13","slug":"evaluation-of-the-microlute-cp-reversed-phase-rp-solid-phase-extraction-96-well-plate-by-hplc-ms","status":"publish","type":"post","link":"https:\/\/clinlabint.com\/evaluation-of-the-microlute-cp-reversed-phase-rp-solid-phase-extraction-96-well-plate-by-hplc-ms\/","title":{"rendered":"Evaluation of the Microlute\u00ae CP Reversed Phase Solid Phase Extraction 96 well-plate by HPLC-MS"},"content":{"rendered":"
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\r\n\"Bio-Rad<\/a>\r\n<\/p>\n<\/div><\/section><\/div>

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Evaluation of the Microlute\u00ae CP Reversed Phase Solid Phase Extraction 96 well-plate by HPLC-MS<\/h1>\/ in White Papers<\/a> <\/span><\/span><\/header>\n<\/div><\/section>
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BACKGROUND<\/strong><\/h4>\n

Solid phase extraction (SPE) is a sample preparation method for the clean-up of samples before chromatographic analysis such as HPLC, and GC. SPE offers a number of advantages to the analyst, including less system downtime and troubleshooting, cleaner chromatograms with a reduction of contaminating compounds, and greater reproducible analyte recoveries. Traditionally, SPE methods use loose-filled resins which often create problems such as voids in the sorbent beds leading to channelling and inconsistent flow-through of solutions. This leads to reduced interactions between analytes and the active resin thus leading to inconsistent results and poor analyte recovery.<\/strong><\/p>\n

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\"Porvair<\/a><\/p>\n

A Porvair Sciences Application Note<\/h5>\n

The Microlute\u00ae CP SPE range from Porvair Sciences, offers a unique, solid hybrid polymer structure which is made up of an interconnected network of evenly distributed pores combined with the retentive media (Figure 1). This design enhances the flow-through of samples to maximise interactions between analytes and the solid phase to deliver a highly reproducible SPE method.<\/p>\n

This application note demonstrates the robust SPE LC-MS methodology of the Microlute\u00ae CP Reverse Phase (RP) SPE 30 mg plates on a range of neutral, acidic, and basic analytes. In addition, comparing recoveries and reproducibility will highlight the performance benefits over loose-filled methods of SPE.<\/p>\n