{"id":19835,"date":"2023-07-06T15:19:38","date_gmt":"2023-07-06T15:19:38","guid":{"rendered":"https:\/\/clinlabint.com\/?p=19835"},"modified":"2023-07-06T15:45:15","modified_gmt":"2023-07-06T15:45:15","slug":"biotium-introduces-novel-antibody-labelling-kits-for-rapid-labelling-of-igm-antibodies","status":"publish","type":"post","link":"https:\/\/clinlabint.com\/biotium-introduces-novel-antibody-labelling-kits-for-rapid-labelling-of-igm-antibodies\/","title":{"rendered":"Biotium introduces novel antibody labelling kits for rapid labelling of IgM antibodies"},"content":{"rendered":"
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\r\n\"Bio-Rad<\/a>\r\n<\/p>\n<\/div><\/section><\/div>

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Biotium introduces novel antibody labelling kits for rapid labelling of IgM antibodies<\/h1>\/ in Product News<\/a> <\/span><\/span><\/header>\n<\/div><\/section>
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Biotium has released Mix-n-Stain CF Dye IgM antibody labelling kits. These kits revolutionize the labelling process for IgM antibodies, enabling researchers to easily obtain high-performance fluorescent IgM conjugates with minimal hands-on time with no purification after labelling.<\/strong><\/p>\n

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IgM antibodies offer advantages over IgG anti-bodies due to their increased avidity. However, traditional methods for conjugation of IgG anti-bodies are frequently ineffective for optimal labelling of IgM antibodies due to differences in the immunoglobulin structures. Recognizing this roadblock, Biotium has successfully opti-mized the reaction conditions and developed these kits to address this issue.<\/p>\n<\/div><\/section>
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Mouse anti-beta tubulin clone 5H1 (IgM kappa) was labeled with CF\u00ae488A using Mix-n-Stain\u2122 IgM labeling, and then was used to stain methanol-fixed HeLa cells at 5 ug\/mL (right panel). The conjugate shows comparable staining specificity compared<\/em>
\nto unlabeled antibody at 1 ug\/mL detected using CF\u00ae488A Goat Anti-Mouse IgM Mu chain secondary antibody at 2 ug\/mL (left panel).<\/em><\/p>\n<\/div><\/section>
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With these kits, researchers can now label 25 ug or 100 ug of their IgM antibody in as little as 15-30 minutes, ensuring rapid experimental workflows. These kits offer unparalleled convenience, with less than 30 seconds of hands-on time required.<\/p>\n

Mix-n-Stain CF Dye IgM antibody labelling kits are available in a selection of 8 CF dyes and FITC. CF dyes are Biotium\u2019s next-generation fluorescent dyes designed to provide superior brightness and photostability compared to other commercially available fluorescent dyes.<\/p>\n

Features and benefits<\/h4>\n

\u2022 Optimized for IgM: Developed to offer high-performance IgM conjugates
\n\u2022 Quick and easy: Conjugate in as little as 15-30 minutes, no purification required
\n\u2022 Wide selection: Available in your choice of 8 bright CF dye colours and FITC<\/p>\n

Digital issue: Please click here<\/a> for more information<\/em><\/strong><\/p>\n

Dr Lori Roberts, Director of Bioscience at Biotium, said: \u201cThis technology provides a new and simple solution to the longstanding challenge of IgM conjugation. We believe these kits will provide researchers with more flexibility in panel design for immunofluorescence and flow cytometry.\u201d<\/em><\/p>\n

For more information, visit: https:\/\/biotium.com\/product\/mix-n-stain-cf-dye-igm-antibody-labeling-kits<\/a><\/em> <\/strong><\/p>\n<\/div><\/section>
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