Page 98 – Clinical Laboratory int.

Biomarkers and preanalytics in Alzheimer’s disease

, 26 May 2023/in Featured Articles /by panglobal

by Dr Jacqueline Gosink Biomarkers and preanalytics in Alzheimer’s disease Analysis of biomarkers such as amyloid-beta peptides and tau proteins in cerebrospinal fluid (CSF) is nowadays a core criterion in Alzheimer’s disease diagnostics. Test methods such as ELISA and chemiluminescence immunoassay enable robust and reproducible measurement of the analytes in patient CSF samples. Recommendations for […]

Figure 1. Respondents gained a variety of INTEGRA pipetting solutions during the pandemic to handle high sample numbers

Responding to a global health crisis: the evolution of the lab

, 26 May 2023/in Featured Articles /by panglobal

by Dr Éva Mészáros Responding to a global health crisis: the evolution of the lab The COVID-19 pandemic forced life sciences laboratories all over the world to suddenly begin high volume PCR testing, to help gain the upper hand over this deadly disease. Many facilities had to adopt new pipetting platforms at short notice to […]

Four saliva samples containing SARS-CoV-2 RNA were mixed with target-specific capture oligonucleotides attached to magnetic beads. The solutions were heated to 95°C for 1 minute, then placed in a rack with a magnet. After 3 minutes the supernatants were replaced with a 1-step RT-qPCR master mix and subjected to a rapid cycling protocol (1 second at 95°C/1 second at 65°C). The same concentration of four pure RNA samples were amplified at the same time and acted as positive controls. the RNA extracted from saliva recorded the same quantification cycles (blue) as the untreated control RNA (red).

Remodelling qPCR as a tool for molecular diagnostics

, 26 May 2023/in Featured Articles /by panglobal

by Professor Stephen Bustin Remodelling qPCR as a tool for molecular diagnostics PCR is an incredibly powerful tool in the field of molecular diagnostics, and its current role is embedded in the clinical laboratory where it is indispensable for non-time-critical routine testing. However, point-of-care testing via lateral flow devices, as seen in the recent […]

Safety of blood and plasma products from pathogen transmission

, 25 May 2023/in Featured Articles /by panglobal

‘The Expert Report to the Infected Blood Inquiry: Statistics’ (http://bitly.ws/EAng) found that around 3650 people with bleeding disorders in the UK were infected with HIV or hepatitis viruses through the use of contaminated blood products. In addition, many people (8120) were found to be chronically infected with hepatitis C 10 years or more after receiving […]