StarBright Violet 760, StarBright UltraViolet 575, and StarBright UltraViolet 605 dyes conjugated to highly validated antibodies for multicolour panel design in immunology research

Bio-Rad Laboratories, a global leader in life science research and clinical diagnostic products, has extended its range of StarBright dyes to provide greater flexibility in multicolour flow cytometry panels. The StarBright Violet 760, StarBright UltraViolet 575, and StarBright UltraViolet 605 Dyes offer improved brightness with narrow excitation and emission profiles for precise resolution.

The Bio-Rad range of StarBright dyes provides researchers with highly stable, validated flow antibodies conjugated to proprietary fluorescent nanoparticles and specific to key immunophenotyping targets, supporting multiplex flow cytometry panels in immunology research. Minimal lot-to-lot variation ensures reproducible and consistent staining, and the dyes are resistant to photobleaching and do not lose signal in fixation. The dyes, excited by violet (405 nm) and ultraviolet (355 nm) lasers, are compatible with the Bio-Rad ZE5 Cell Analyzer and S3e Cell Sorter, as well as most flow cytometers and a variety of experimental protocols, without the need for special buffers.

“The continued expansion of the Bio-Rad StarBright Dye portfolio, currently totalling 17 dyes, nine of which are StarBright violet dyes, gives researchers greater flexibility and choice when designing flow cytometry panels, by providing high-performance dyes excitable at a range of wavelengths,” said Mike Blundell, PhD, Bio-Rad Product Manager, Flow Cytometry, Life Science Group. “The exceptional brightness and unique profile of the dyes delivers enhanced resolution of rare populations and low-density antigens.”

• To learn more about Bio-Rad StarBright dyes, visit: bio-rad-antibodies.com/flow-cytometry-starbright-dyes.

London-based PCR Biosystems has launched Air-Dryable Probe 1-Step Mix, a versatile all-in-one, air-dryable 4x RT-qPCR reagent mix for ultra-sensitive detection of RNA and DNA sequences. With this kit, the robust performance of the company’s liquid PCR reagents can be brought to dried assay formats using a standard laboratory oven, offering a simpler, faster, and cheaper alternative to lyophilisation (freeze-drying).

Now, a larger base of assay kit developers, including smaller organisations with fewer resources, can access the advantages of dried assays — shorter time-to-market, simplified logistics and storage, and significantly reduced costs. Sectors set to realise such benefits include clinical, veterinary, food, environmental and agricultural testing.

Assay developers moving toward dried test formats

The costly cold-chain shipping and storage demands of traditional ‘wet’ RT-qPCR reagents and tests are increasingly driving assay developers toward dried test formats. But while lyophilised assays have emerged as a powerful option that can reduce test weight, volume, and transport and storage requirements, they can be out of reach for smaller, resource-strapped organisations. This is because lyophilisation requires expensive, specialised equipment, deep technical expertise, and extensive drying times. Outsourcing lyophilisation alleviates some of this burden, but it entails loss of process control.

With the new air-dryable kits, drying can be done in just 80 minutes with a simple laboratory oven, without extensive training, and at a much lower cost. As a result, organisations unable to build onsite lyophilisation capabilities can now create high-performance dried assays in-house, with full control over the drying process and quality control steps. The new kits also do not suffer from the restrictive drying protocol rigidity that can often complicate lyophilisation. Further, air-dried assays are less susceptible to moisture and so do not require the strict humidity control during manufacturing that is needed with lyophilised tests. This is particularly useful in high-humidity regions and allows local diagnostic kit developers to more confidently expand their portfolio into dried formats.

As with the company’s lyophilisable mixes, the air-dryable mixes retain performance after reconstitution. Kits also come with the company’s renowned technical support to ensure smooth troubleshooting and optimal results, and free samples are available.

“Dryable reagents are redefining the ease and convenience with which PCR assays are being developed, distributed, and used. Lyophilisation has played a huge part here, but barriers to its adoption have seen smaller companies get left behind,” said Alex Wilson, co-founder of PCR Biosystems. “Our air-dryable mixes erode barriers to drying, so even with minimal resources, developers can get superior-quality dried assays to the point of care, efficiently. This is the latest step in our journey to place simple, cost-effective, and high-performing tests within reach of all.”

What’s in the Air-Dryable Probe 1-Step Mix kit?

The new kits comprise a glycerol-free 4x qPCR mix with hot start Taq polymerase, dNTPs, MgCl₂ and a blend of excipients for reliable drying without activity loss. A more stable version of UltraScript Reverse Transcriptase and RiboShield RNase inhibitor are blended into the mix, providing a more convenient single-tube format. Kits can be used for both RNA and DNA detection for greater versatility, and dried assays are stable for 3 months at 37°C.

Critically, PCR Biosystems is certified to conform to Quality Management System standard ISO 13485, ensuring full product traceability, and optimum lot-to-lot consistency. Assay developers can therefore be confident in using the new air-dryable kits to create the highest-quality tests.

• For more information, visit: Air-Dryable Probe 1-Step Mix
• Free samples can be requested via this form.

 

New Hampshire Public Health Laboratories (NHPHL) has adopted INTEGRA Biosciences’ ASSIST PLUS pipetting robot and MINI 96 portable electronic pipette to automate part of its influenza and SARS-CoV-2 testing process. These convenient solutions have helped the lab to increase its throughput and tackle a sample backlog despite the ongoing turmoil caused by the global pandemic.

Porvair Sciences has developed a 2 ml 96-well microplate designed for labs processing cannabis samples for analysis of Tetrahydrocannabinol (THC) and Cannabidiol (CBD).

Sample processing is the second step in testing cannabis strains. This is carried out after collecting leaf, bud, and flower samples from cannabis plants, ready for accurate determination of the THC/CBD ratio by HPLC.

Traditionally sample processing protocols have used bead-beating or grinding mills to homogenize the plant materials. When this sample processing is carried out in standard polypropylene microplates, the extreme forces applied by these machines can cause damage to the plates, leading to cracking, leaking, and incorrect results due to sample cross-contamination.

To address these design shortfalls, Porvair Sciences developed a reinforced microplate that could withstand the applied forces in bead beaters while maintaining a standard ANSI/SLAS footprint making the methodology automation compatible.

Based upon a 2 ml square well block design, Porvair Sciences cannabis sample processing microplate sets a new standard for reliable, high productivity cannabis plant processing. To ensure that no contamination from the plate will affect the THC/CBD analysis, Porvair Sciences only uses pure, extractables-free polypropylene, to manufacture its plates.

• For more information, visit; Porvair’s 96-well microplate designed for labs processing cannabis samples