Immunochromatographic strip for STD pathogen detection

Operon has developed a new molecular diagnostic line based on the detection of amplified PCR products by immunochromatographic strip: the OligoGen products. This technology detects DNA from several pathogens associated with sexually transmitted diseases (STD) such as Chlamydia trachomatis, Neisseria gonohrroeae, Herpes simplex, Mycoplasma genitalium and more. The general procedure has three steps: DNA extraction, amplification and hybridization/detection. During this hybridization step, the specific DNA fragments bind to probes covalently bounded to latex coloured particles (colloid). Subsequently, during immunochromatography, these DNA complex + colloid are detected by specific antibodies that recognize labels added during PCR, resulting in the appearance of a coloured band pattern. The main advantages of these products include ready to use reagents provided in the kits, minimal equipment requirements and a hybridization step carried out in a tube, which increases the specificity and reproducibility of the process.

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C064 Vela Diagnostics

Direct MRSA/SA PCR test

Methicillin resistant Staphylococcus aureus (MRSA) infections are responsible for a high number of patient deaths in hospital settings and significantly contribute to health care costs. The reduction of healthcare-associated MRSA infections could potentially improve patient outcome and reduce the level of service, provided test results can be turned around in an adequate time and testing is cost effective. The Sentosa SA Direct MRSA/SA PCR test is a rapid PCR 4-plex assay for the simultaneous detection and differentiation of S. aureus Type I to VII and XI SCCmec/orf X cassettes including mecC. Test results are available after only 2.5 hours with minimum hands-on time due to automated reporting. Differential target detection reduces the number of false positive MRSA results caused by cassette “empty” mecA or S. epidermidis infections. The reliable and cost efficient Sentosa SA Direct MRSA/SA PCR test is well suited for today’s healthcare settings and an excellent alternative to culture methods or more cost-intensive PCR tests.

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Rapid test for Legionella pneumophila

Legionellosis is a serious pneumonia caused by bacteria of the genus Legionella (family Legionellaceae). Legionella bacteria are readily found in fresh water, cooling towers and potable water systems. Cough can be the first sign of infection; other common symptoms include headaches, muscle aches, chest pain and shortness of breath. Gastrointestinal symptoms are also common. Legionnaires’ disease is transmitted by aerosol, and there is no evidence for direct person-to-person transmission. Diagnosis of legionellosis can be difficult because signs and symptoms are non-specific and do not distinguish L. pneumophila infections from other common causes of pneumonia. One of the most common diagnostic methods is the detection of urinary antigen in urine. Urinary antigen is detected as early as one day after onset of symptoms and remains detectable for days or even weeks. This technique combines sensitivity and high specificity with rapid results. The Legionella V-TesT detects soluble antigen from L. pneumophila serogroup 1 in urine. The test was evaluated on 300 samples in a reference lab for respiratory diseases and demonstrated a sensitivity of 97.6 % and a specificity of 97.7 %.
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Seamless, one-piece serological pipettes

Available in 1mL, 2mL, 5mL, 10mL and 25mL volumes, Globe Scientific’s new plastic serological pipettes are manufactured as a single piece. Unlike other brands in which separate tip and mouth pieces are welded to the main body, the company’s one-piece seamless design eliminates the weld points that trap liquid. This unique design provides faster fill and release rates, ensures a complete flow out of the sample and offers a higher degree of accuracy. Each pipette has an aerosol barrier plug to prevent both liquid and aerosols from contaminating the pipettor. These serological pipettes are produced from crystal clear polystyrene and are colour coded for easy volume identification. The calibration of the pipettes is certified for accuracy. Sterile pipettes are RNase-free, DNase-free, non-pyrogenic, non-haemolytic and non-cytotoxic.
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C143

Test to distinguish acute from past infection with Toxoplasma gondii

The Toxoplasma gondii IgG Avidity EIA test from Ani Labsystems has been updated. New T. gondii IgM EIA and IgG EIA tests have also been developed to form a full Toxo package. The new T. gondii IgM EIA is a capture test that can be run simultaneously with the new T. gondii IgG EIA. The T. gondii IgG EIA test provides quantitative results, and automatically generates the guidelines for the avidity assay, which needs only two dilution points. The T. gondii IgG Avidity EIA kit contains avidity wash buffer and avidity controls, and the kit is sold as a convenient supplementary package to the IgG test. The strips of kits have been individually labelled for easy identification. The tests can be automated or run manually.
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Sexually transmitted infection array

Sexually transmitted infections (STIs) present an increasing challenge to healthcare systems worldwide. Early and correct diagnosis is important as STIs can lead to other significant health problems including infertility, many infections are asymptomatic and remain undiagnosed, and undiagnosed STIs can increase the risk of uncontrollable spread. Allowing rapid, simultaneous detection of ten sexually transmitted infection pathogens from urine samples or urogenital swabs, the Randox STI array is a highly sensitive and specific PCR amplification assay coupled with Biochip Array technology. Rapid multiplexing capbility is provided with results available in less than three hours. The pathogens detected include: Chlamydia trachomonas; Neisseria gonorrhoea; Trichomonas vaginalis; Treponema pallidum; Herpes simplex I; Herpes simplex II; Mycoplasma hominis; Mycoplasma genitalium; Ureaplasm urealyticum and Haemophilus ducrey.
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Automated fluorometric plate reading system

Developed for use with Sensititre microtitre plates, the Sensititre OptiRead automated fluorometric plate reading system combines fluorescence technology with the powerful SWIN software system. MIC plates can be quickly and accurately read, maximising consistency and eliminating manual reading. The system provides user-friendly automation for busy laboratorians, rapidly transferring test results for processing, interpretation and report generation, and improving efficiency and productivity. The instrument’s compact design conserves valuable laboratory space, operating as either standalone, or in combination with the fully automated ARIS 2X reading and incubation system, replacing the current AutoReader instrument.
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Point-of-Care blood analysis system

The newest version of Alere’s epoc Blood Analysis System is available. A cost-effective, point-of-care testing solution that makes use of Smartcard technology and wireless connectivity, the system provides real-time, lab-quality blood gas, electrolyte and metabolite results at the patient’s bedside. Testing that is run through a central lab can typically take up to 40 minutes to complete, but, with this platform, clinicians are able to access results immediately and make vital treatment decisions, all while minimising operational costs. Hosted on a new handset, the new system has even more functionality than its predecessor. Platform enhancements include a built-in 2D barcode, alpha-numeric keyboard and improved connectivity. The Microsoft Windows Mobile Operating System has also been upgraded, making the platform even faster, allowing for more robust use in a critical care environment, and optimising screen resolution so results can be easily read.
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Chemiluminescence analyser

Suitable for any autoimmune laboratory, and offering precise quantification and a broad dynamic range using chemiluminescent technology, the BIO-FLASH is a fully automated, random access chemiluminescent analyser. The instrument provides enhanced workflow efficiencies, ease-of-use and improved assay performance compared with existing enzyme-based systems. With its stable on-board reagents, which allow for samples to be run as they arrive, and its stored calibration curves, even the most specialised autoimmune tests are efficient to perform. The instrument has a through-put of up to 64 tests per hour, with results available in as little as 30 minutes.
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Trefoil Factor 3 (TFF3) ELISA

Human Trefoil Factor 3 (TFF3) belongs to the trefoil factor family that also includes Trefoil Factors 1 and 2. It is mainly expressed in gastrointestinal tract, and in the mucous layer of the small and large intestine. Kidney is another major site of TFF3 expression. Overexpression of TFF3 has been reported in patients with various neoplasms including intestinal, pancreatic and prostate carcinomas. It is typically expressed in the lower oesophagus undergoing intestinal metaplasia. Urinary TFF3 protein levels are markedly reduced in response to renal tubular injury. Higher urinary TFF3 levels indicates ongoing repair of damaged kidney. Urinary TFF3 can be a marker of kidney disease. The Human TFF3 ELISA has enhanced BioVendor’s portfolio of diagnostic kits for renal disease, oncology, immune response, infection and inflammation, and metabolic syndrome. The kit is suitable for use with serum, plasma, urine and bronchoalveolar lavage samples.
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