The MERGITE! device from EUROIMMUN increases the efficiency and reproducibility of indirect immunofluorescence tests (IIFT) by providing automated washing of incubated slides. In the high-throughput washing procedure, up to 50 substrate fields are washed in parallel in approximately 40 seconds (instead of 5 minutes with manual washing). The direct and controlled liquid flow provides fast, meticulous washing which is gentle on the substrates. Cross contamination is prevented by separate washing of each field. The standardized, predefined wash programmes ensure a consistent washing quality and high reproducibility. Since manual drying of slides is no longer necessary, the risk of damage to the substrates is substantially reduced. The automated washing procedure reduces wash buffer usage by up to 50% compared to manual washing and eliminates the need for beakers, cuvettes and paper towels. Specially developed carriers provide efficient and safe handling of slides during incubation, washing and covering. The device has an intuitive user interface for easy use and minimal familiarization time. Its integrated touch screen enables independent operation without connection to a PC. The compact tabletop device requires very little standing space in the laboratory. MERGITE! is part of a complete package from EUROIMMUN for fully automated processing, microscopy and evaluation of IIFT.

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Beckman Coulter Life Sciences has opened up the possibilities for cancer researchers with the launch of the first flow cytometer to offer excitation sources across the visible spectrum in a standard configuration. The CytoFLEX LX Blue-Red-Violet-Yellow Green-Ultraviolet-Infrared series incorporates excitation sources from ultraviolet 355 nm to infrared 808 nm alongside the existing Avalanche photodiode detectors (APD).  For the first time, this allows researchers to utilize the periphery of the visible spectrum. The CytoFLEX provides powerful analytical performance in a compact, benchtop instrument.  It leverages the effective use of Avalanche photodiode detectors to deliver the instrument’s advanced sensitivity. They work in conjunction with the different lasers, detecting the light emitted from the cells after excitation.  The addition of the new ultraviolet laser further expands the applications available to the researcher. While flow cytometry is recognized as a powerful technique for single cell analysis, traditionally it has focused on the centre of the visible light spectrum. The availability of the UV excitation source opens up research into cell-cell interactions under both normal and abnormal conditions.  For example, this will enable investigators to obtain separation of non-stained auto-fluorescing populations from positively stained populations. At the other end of the spectrum lie the infrared wavelengths, 808 nm. This is a field where more and more detectors are packed into an already crowded space.  The opening up of the infrared end of the spectrum sees an exciting expansion of the capabilities of flow cytometry.  Biologists will have space in the spectrum to simplify complex experiments, helping them tease apart subtle, but potentially meaningful, variations in their cellular analyses. The decision to incorporate Avalanche photodiode detectors, not available in other flow cytometers, in the design of the original CytoFLEX makes this expansion possible.  With their high and stable quantum efficiency from 400 to 1100 nm, they can enhance performance when using the new infrared laser.  In addition, the CytoFLEX includes an extensive set of repositionable band pass filters, the flexibility to upgrade by adding additional parameters and intuitive software to facilitate multicolour analysis.  

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Hologic has obtained a CE mark in Europe for its new Panther Fusion system and Panther Fusion assays for flu and respiratory testing. The Fusion module system offers laboratories the ability to extend the testing capabilities of the existing Panther system. The Panther Fusion module is an in‐lab upgrade to the Panther instrument. This addition adds the flexibility of PCR (polymerase chain reaction) to the proven performance of TMA (transcription-mediated amplification), which is used by the Panther system. Offering labs multiple chemistries on a single platform enables further consolidation of their testing portfolio. The Panther Fusion system retains all the key benefits of the Panther platform, including fully automated sample-to-result automation, multiple tests from a single sample, random access sample processing, continuous loading, and STAT capabilities. Additional benefits include a higher throughput of up to 335 Panther Fusion tests in eight hours, or up to 500 Fusion and Aptima tests. Flexibility is also improved with the addition of open access functionality to run laboratory-developed tests and protocols. This new capability allows laboratories to concentrate on developing assays, while the Panther Fusion system automates workflow and reduces overall hands-on-time. The addition of the flu and respiratory assays to the broad menu of Aptima tests already available for the Panther instrument further extends the range of diagnostic tests that laboratories can run on one Hologic system. The respiratory panel assays include the Panther Fusion Flu A/B/RSV, Panther Fusion Paraflu, and Panther Fusion AdV/hMPV/RV tests. The respiratory assays offer a modular approach to syndromic testing via the ability to run one, two or all three assays from a single patient specimen depending on the needs of a given patient. The new Panther Fusion PCR assays, combined with the Aptima TMA assays, make the Panther Fusion system the only instrument worldwide that combines TMA, real-time TMA and real-time PCR with full sample to result automation. The Panther Fusion system and Panther Fusion assays have not been cleared for marketing by the U.S. Food and Drug Administration, and are not available for diagnostic use in the United States.
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Carnitine/acylcarnitines screening is the most important part of the clinical laboratory diagnosis of amino acid, fatty acid or organic acid metabolism disorders. Carnitine / acylcarnitines screening allows the diagnosis of over 30 metabolic diseases from a drop of blood sample taken with a screening card. This screening test is performed by measurement of carnitine / acylcarnitines with isotope dilution tandem mass spectrometry. LC-MS/MS is a powerful tool that gives results for carnitine / acylcarnitines in 1.5 minutes. The screening can be performed by the Zivak Carnitine / Acylcarnitine LC- MS/MS Analysis Kit and Zivak NeoZin screening software. The main methods and procedures that have been selected are based on EN ISO 14971. The quantitative analysis of carnitine esters including free carnitine, C0, C2, C4, C6, C8, C10, C12, C14, C16, C16 OH, C18, C18 OH carnitine reveals disorders which are related to fatty acid oxidation. The quantitative analysis of carnitine esters including C3, C3DC, C4OH, C5, C5OH, C5DC carnitine reveals disorders which are related to organic acid metabolism.

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