2-manipulation technique for SNPs detection without DNA extraction
The LAMP Human FII&FVL Duplex KIT (rs1799963/rs6025) allows every lab to perform the qualitative detection of the Prothrombin G20210A mutation (rs1799963) and the Factor V Leiden mutation (rs6025) by Loop-mediated isothermal amplification (LAMP) in an easy, fast and cost-effective way. LaCAR offers a wide panel of SNP detection kits requiring only two manipulations and without DNA extraction. The whole process including amplification, reading and results interpretation can be performed in approximatively 30 minutes. Every product developed by LaCAR has been validated and CE-IVD marked on its LC-Genie III (compact device for amplification, reading and results interpretation – see photo) but also on various high throughput platforms such as LightCycler 480 (Roche), LightCycler 96 (Roche), CFX96 (Biorad)… LAMP is a relatively new DNA amplification technique, using an isothermal nucleic acid amplification, which eliminates the need for expensive thermocyclers used in conventional PCR or qPCR and could provide major advantages due to its simplicity and ruggedness. In LAMP, the target is amplified at a constant temperature (+/- 65°C) using three sets of primers and a polymerase with high strand displacement activity. Detection of the different genotypes is performed by melting curve analysis after amplification. Both mutations are detected simultaneously by using a different fluorescent colour for each mutation.
