AMSBIO unveils rapid immunofluorescent staining kit for 3D cell cultures
Accelerating organoid and spheroid research
AMSBIO has unveiled CellO-IF, a novel all-in-one immunofluorescent staining reagent kit designed to revolutionise the labelling of organoids and spheroids. This innovative product promises to significantly reduce the time required for immunofluorescence (IF) staining, potentially transforming workflows in laboratories studying three-dimensional cell cultures.
Traditional IF staining techniques, while offering excellent sensitivity and signal amplification compared to immunohistochemistry, are notoriously time-consuming and labour-intensive. CellO-IF addresses these challenges by enabling direct staining of samples within hydrogels or extracellular matrices, thereby preserving delicate structures and cellular integrity.
Streamlined protocol
The kit eliminates several steps in the conventional IF process, including harvesting, clearing, transferring, and centrifuging. This streamlined approach not only accelerates the staining procedure but also minimises sample loss, a common issue in 3D cell culture research.
CellO-IF boasts improved antigen visualisation capabilities, routinely producing high-resolution images. Interestingly, the kit has demonstrated success in labelling samples with primary antibodies that were previously ineffective using traditional methods, potentially expanding the range of antigens that can be studied in 3D cultures.
Optimised for both 3D and 2D applications
AMSBIO offers CellO-IF in two formats, optimised for either 3D or 2D samples. Both versions are designed to enhance signal intensity, facilitating clearer visualisation of targets of interest and potentially leading
to more accurate research insights.
The introduction of CellO-IF represents a significant advancement in IF staining technology, offering researchers the ability to obtain high-quality data from complex 3D cell cultures within hours rather than days. This development could accelerate research in fields such as cancer biology, stem cell research, and drug discovery, where organoids and spheroids are increasingly used as physiologically relevant models.
For more information, visit: https://www.amsbio.com/3d-cell-culture-extracellular-matrices/celloif
Digital issue: Please click here for more information
