Azenta Life Sciences launches low DNA binding consumables range for NGS and molecular biology workflows
Azenta Life Sciences has introduced a range of low DNA binding consumables designed to minimise nucleic acid loss during sensitive applications, including next-generation sequencing (NGS) library preparation and molecular biology workflows where low DNA input is required.
Addressing sample loss at low nucleic acid concentrations
As NGS workflows increasingly involve smaller quantities of DNA and RNA, the interaction between nucleic acids and plastic consumable surfaces has become a more significant consideration. Whilst polypropylene is a standard material for PCR and sequencing applications owing to its chemical resistance and thermal stability, adsorption of nucleic acids to plastic surfaces can become problematic at low concentrations, particularly across multiple handling and incubation steps. Azenta’s Low DNA Binding range uses carefully selected polypropylene polymers formulated to reduce these surface interactions, with the aim of delivering more consistent recovery and reproducible results in downstream assays.
Product range and formats
The consumables portfolio includes FrameStar® PCR plates in 96- and 384-well formats. These feature thin-walled polypropylene wells to facilitate efficient thermal transfer, combined with rigid polycarbonate frames to support accurate robotic handling and stable thermal cycling. Extra-rigid skirted designs are also available for high-throughput systems where plate alignment and stability are critical parameters. The range is further supplemented by low binding storage and deep well plates suited
to common NGS preparation steps, including magnetic bead-based clean-up workflows.
Quality certification and performance data
All products within the range are certified free from human genomic DNA, nucleases, and pyrogens, and undergo rigorous quality testing including leak testing and PCR performance verification.
Avash Anderson, Product Manager at Azenta Life Sciences, commented: “Azenta’s Low DNA Binding consumables deliver the quality and consistency needed for reliable molecular biology workflows. All products are certified free from human genomic DNA, nucleases, and pyrogens, and undergo rigorous testing – including leak testing and PCR performance verification – giving laboratories confidence in even the most sensitive applications.”
In an independent comparative study, Azenta’s low binding PCR plates showed no significant DNA loss following incubation, whereas other commercially available low binding plates demonstrated measurable DNA loss at low starting concentrations, as indicated by increased Ct values.
Azenta, Inc. is headquartered in Burlington, Massachusetts, with operations across North America, Europe, and Asia, and markets its products under brands including GENEWIZ, FluidX, and 4titude.
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