Infections by carbapenemase-producing Enterobacteriaceae (CPE) are the cause of major public health concern worldwide and their detection is very challenging for diagnostic laboratories. Classical identifications need 48 to 72 hours with cultures and susceptibility testing followed by phenotypic and molecular methods. Development of new rapid diagnostic tests to track antimicrobial resistance patterns is considered as a priority core action. Non-molecular confirmatory testing of Class A (KPC) and B (VIM, IMP, NDM) carbapenemases already exists but not for detection of Class D carbapenemase (OXA-48-like). Phenotypic colorimetric assays are not able to confirm OXA-48 presence, while molecular tests need a dedicated environment, skilled staff and are expensive. Coris BioConcept has developed a new assay for identifying OXA-48 carbapenemase in collaboration with the CPE Belgian National Reference Centre of CHU Dinant-Godinne UCL. This test is performed directly from a single colony and represents the first non-molecular confirmatory assay for OXA-48 detection with results available in minutes.