Siemens Healthineers is launching to laboratories Sysmex’s CN-3000 and CN-6000 Hemostasis Systems for mid- and high-volume coagulation testing. The company offers a broad menu of both routine and specialty coagulation assays that help identify blood coagulation disorders that can be performed on the Sysmex CN-3000 and CN-6000 Systems. This includes the new Siemens Healthineers’s INNOVANCE Anti-Xa assay for heparin and direct oral anticoagulant testing (DOAC).
“Siemens Healthineers and Sysmex’s 25-year partnership in hemostasis enables us to deliver both quality and comprehensive hemostasis testing solutions to laboratories of all sizes across the world,” said Deepak Nath, PhD, President of Laboratory Diagnostics for Siemens Healthineers.
INNOVANCE Anti-Xa assay
Siemens Healthineers’s new INNOVANCE Anti-Xa assay is a ready-to-use, liquid-reagent heparin and DOAC assay. It delivers precise results without reagent reconstitution or waiting before use. The assay streamlines handling, reduces ordering complexity by enabling labs to test for heparin, as well as direct oral anticoagulants rivaroxaban and apixaban with one combined assay, and offers labs an economical testing solution for heparin and DOAC testing.
Atellica Process Manager
The Sysmex CN-3000 and CN-6000 Systems are designed to integrate with the Siemens Healthineers Atellica Data Manager and Aptio Automation to further streamline a hands-free workflow in the laboratory and to automate sample processing – including sample preparation as well as storage, refrigeration, and retrieval. With the ability to connect to Atellica Process Manager, labs can manage testing processes from a centralized location, while also gaining access to analytics to baseline and track key performance indicators such as turnaround time or problem samples. When connected to Atellica Data Manager, advanced algorithms can automatically determine the next steps in sample processing and routing helping labs to standardize testing protocols across multiple systems and locations.
Designed to be simple to operate, the new Ultraseal LITE semi-automatic microplate sealer from Porvair Sciences is the perfect device for labs with low to medium throughput (6 plates / minute) processing needs.
Compact in design, the Ultraseal LITE will fit just about anywhere you need to use it – be it on a lab bench or inside a fumehood.
Combining a powerful 400W heater with a strong sealing action, the Ultraseal LITE is able to ensure high integrity sealing of almost any type of microplate including deep well, shallow well, low profile deep well, filter and assay plates that conform to the ANSI / SLAS standard format.
The Ultraseal LITE utilises heat seal films and foils to give either permanent or peelable seals for all common microplate material types (polypropylene, polystyrene, polyethylene).
A typical sealing time for most polypropylene plates using Porvair seals is less than 3 seconds each. Seal times and seal temperatures are fully adjustable using the Ultraseal LITE control touch screen. Sealed plates are automatically ejected from the Ultraseal LITE.
The Ultraseal LITE has dual voltage capability, allowing it to operate on both 120 and 240 volt alternating current, 50/60 Hz mains supplies without the need for a power transformer.
Waters Corporation has introduced the Waters™ SELECT SERIES™ MRT, a high-resolution mass spectrometer that combines Multi Reflecting Time-of-Flight (MRT) technology with both enhanced DESI and new MALDI imaging sources. Together, these technologies enable scientists to explore molecular structure and function through the precise identification and localization of individual molecules in samples, with a unique combination of speed, resolution, and mass accuracy.
For example, a scientist researching how an investigational oncology drug interacts with its intended target, such as a specific brain tumour receptor, can now do so much faster, at speeds up to 10Hz without compromising mass accuracy or resolution.
The SELECT SERIES MRT platform is the basis for the next generation of Waters’ high-resolution mass spectrometers. It is the first commercially available instrument of its kind that is capable of attaining 200,000 Full Width Half Maximum (FWHM) resolution and part-per-billion mass accuracy independent of scan speed. The superior performance of the SELECT SERIES MRT allows scientists to get the highest quality mass spectrometry data and information faster than any commercial mass spectrometer on the market todayii. The new Multi Reflecting Time-of-Flight technology features an extended flight path of almost 50 meters to give scientists a much clearer picture of structural information, including fine isotope structure.
“The Waters SELECT SERIES MRT opens the door to a greater understanding of molecules and their mechanisms of action in numerous scientific fields,” explained Dr. Udit Batra, CEO and President, Waters Corporation. “The clarity of images produced by this instrument can yield profoundly important molecular information to scientists in research areas such as targeted therapeutics, to provide insights and enable deep biological discoveries.”
DESI XS and MALDI imaging on a single MRT platform
The first application of the SELECT SERIES MRT unlocks the potential of MS imaging using DESI and MALDI ionization. DESI is a direct-from-sample, soft ionization technique performed under ambient environmental conditions, requiring no prior sample preparation. It provides imaging information about the biochemical distribution of small molecule drugs, lipids, and naturally occurring metabolites within a slice of tissue or on other sample surfaces after just a single measurement. Scientists are applying DESI to research breast and ovarian cancer, to study drug metabolism and pharmacokinetics (DMPK), to investigate the function of key metabolites in natural products in traditional medicines, and to get a closer look at the biotransformation of bacterial colonies after gene modifications.
Featuring a new high-performance sprayer and a heated transfer line, the advanced DESI XS source delivers new levels of usability, image clarity and sensitivity at high speed, bringing more accessible imaging to scientists through mass spectrometry data.
For scientists interested in conducting MALDI imaging experiments, the SELECT SERIES MRT is available with a newly designed MALDI source. MALDI is an ideal complementary technique for the imaging of biological molecules such as peptides and can visualize tissue sections at spatial resolutions of less than 10 microns. Taken together, the combination of imaging sources gives researchers the means to generate fine spatial information and morphological detail for biological samples and even visualize brain vasculature.
Customer shipments of the SELECT SERIES MRT are expected to begin in the fourth quarter of this year.
https://clinlabint.com/wp-content/uploads/sites/2/2021/06/waters.jpg628906panglobalhttps://clinlabint.com/wp-content/uploads/sites/2/2020/06/clinlab-logo.pngpanglobal2021-06-14 11:18:192021-06-14 11:18:19Waters sets new standard in high resolution mass spectrometry with SELECT SERIES Multi Reflecting Time-of-Flight platform
Small molecule researchers in academia, pharmaceutical, and biopharmaceutical industries can now use a new, intelligent mass spectrometer (MS) to reveal complex chemical structures with confidence, ease and experimental versatility.
The Thermo Scientific Orbitrap IQ-X Tribrid mass spectrometer has been designed specifically for small-molecule structural elucidation of metabolites and unknown compounds. The wide range of applications include metabolomics and lipidomics research, leachable/extractable impurities identification and forensic toxicology. The MS system provides a fit-for-purpose, new AcquireX workflow enabled by the trusted Thermo Scientific Tribrid architecture to acquire high-quality data. Performance is enhanced by a new Met-IQ workflow that leverages real-time library search with on-the-fly intelligent MS² spectral matching for decision-based triggering of MSⁿ scan events, providing higher confidence for chemical annotation.
“Along with the enhanced intelligence-based data acquisition, the Orbitrap IQ-X Tribrid MS also uses the new Auto-Ready ion source for automated, remote, and walkaway-calibration enabling the researcher to spend more time obtaining results and less time on instrument setup. Traditionally, ultra-high resolution and accurate mass calibration has been challenging and time consuming. The new system offers researchers higher sample throughput with increased ease-of-use,” said Iain Mylchreest, vice president, research and development, analytical instruments, Thermo Fisher Scientific.
The Orbitrap IQ-X Tribrid MS utilizes enhanced Thermo Scientific AcquireX workflows for increased sample throughput and ease-of-use. The MS system provides improved fragmentation for small-molecule analysis with options to add to the platform’s experimental flexibility. An ultraviolet photodissociation (UVPD) option provides insights on the lipid double-bond localization, site specific glucuronidation, and complementary fragmentation for structure assignment while the 1,000,000 (1M) resolution option improves confidence for unknown analysis and fine isotope detection.
https://clinlabint.com/wp-content/uploads/sites/2/2021/06/thermo_fisher_orbitrap.jpg548888panglobalhttps://clinlabint.com/wp-content/uploads/sites/2/2020/06/clinlab-logo.pngpanglobal2021-06-14 11:14:052021-06-14 11:14:05Thermo Fisher Scientific unveils Mass Spectrometer designed for small molecule analysis
CytoSMART Technologies has launched two new fluorescence live-cell imaging systems – the Lux3 FL Duo Kit and the Multi Lux3 FL. The CytoSMART Lux3 FL Duo Kit is a compact and cost-effective imaging system consisting of two fluorescence microscopes, designed for side-by-side comparison of cell cultures. The CytoSMART Multi Lux3 FL fluorescence live-cell imaging system consists of four compact Lux3 FL devices, equipped with two fluorescent (green and red) and one brightfield channel, and designed for long-term comparative studies and larger research teams.
Both systems operate from within a standard CO2-incubator or hypoxia chamber, allowing to analyse cells in their desired culture environment. Each system of two and four microscopes is connected to a single laptop, maximizing precious laboratory space. Monitoring and analysis of the running experiments can be done at any time from anywhere via the CytoSMART Cloud.
Jan-Willem van Bree, CTO at CytoSMART Technologies, said: “Last year we launched our first fluorescence live-cell imager, the CytoSMART Lux3 FL, and our customers were pleased with its performance and expanded research possibilities. This year we decided to scale up fluorescence live-cell imaging possibilities by launching two new advanced systems: the Lux3 FL Duo Kit and the Multi Lux3 FL. We always aim to develop and offer versatile and flexible solutions to life scientists. For example, the Multi Lux3 FL allows to run up to four individual imaging experiments simultaneously, without affecting the time-lapse imaging experiments of other lab members. This is a very convenient lab equipment for every research group that studies cell viability, co-culture models, single-cell migration, and many other cell-based assays can benefit from it.”
The main features and benefits of the CytoSMART fluorescence live-cell imaging systems include:
Ideal for comparative studies– directly compare fluorescently-labeled cell cultures.
Versatile – expand the number of variables you can examine using fluorescent labelling.
Flexible – connect devices to the same laptop and control them individually.
Incubator-friendly – study cells in their desired culture environment.
Full remote access via the CytoSMART Cloud – no need to enter the lab to inspect cell cultures.
Indispensable tool for long-term comparative studies – run up to four experiments simultaneously for days or weeks.
Cost-effective and efficient solution – two or four fluorescence imaging devices connected to one laptop, including unlimited storage and unlimited number of user accounts.
BioChromato has developed an enzyme assay product – the RAPID Tube Plate 600 (TP-600) that is helping deliver outstanding results in in vitro metabolic stability studies.
The TP-600 is manufactured from ultra-pure polypropylene that does not contain any enzyme inhibitors such as EDTA or other contaminants that may degrade your assay results. In addition, the TP-600 is guaranteed to be Human DNA, DNase and RNase free. Used with a BioChromato aluminium heating block the TP-600 is designed to provide superior thermal distribution between and in individual tube plate wells ensuring high consistency in results after incubation.
With a wide operating range of -80°C to +130°C the integrity of your TP-600 is unaffected by cryogenic storage or autoclave sterilisation at 121°C.
The TP-600’s ANSI / SLAS compliant footprint and large 600 µL capacity per well provides the flexibility for this enzyme assay tube plate to be used in both manual and automated applications including pharmacokinetic assays, metabolic stability studies, high throughput screening and cold storage.
Bio-Rad Laboratories has introduced three StarBright dyes designed for flow cytometry applications.
StarBright Violet 570, 670, and 710 dyes offer improved brightness and precise excitation and emission spectra, making them suitable for use in multicolour flow cytometry panels. Bio-Rad’s line of StarBright dyes offers researchers a range of fluorescent nanoparticles conjugated to highly validated flow antibodies.
“The new StarBright dyes offer improved brightness when compared to other available dyes at similar wavelengths, and they may be used with a wide range of flow cytometers,” said Mike Blundell, PhD, Bio-Rad Product Manager, Flow Cytometry, Life Science Group. “The unique profile of the dyes offers enhanced resolution of rare populations and low-density antigens as well as consistent and reproducible staining.”
Key benefits of StarBright dyes
Compatible with new and existing protocols and suitable for use in multiplex panels
Resistant to photobleaching and highly stable with minimal lot-to-lot variation
Offer reliable Förster resonance energy transfer (FRET) for reproducible data
Work with common staining buffers, including special polymer dye staining buffers, for easy integration into multicolour panels
Ensure no loss of signal in fixation
Offer narrow excitation and emission profiles for improved resolution in new and existing multicolour flow cytometry experiments
The StarBright Dyes are compatible with Bio-Rad’s ZE5 Cell Analyzer, S3e Cell Sorter, and other conventional and spectral flow cytometers.
3Brain AG has introduced a new technology based on a unique combination of microchips and AI that promises to change the way drug candidates are selected at the preclinical level to enter into clinical trials – and to maximize their chances of success.
Using CorePlate™ technology, preclinical cell assays are performed in multiwell plates that integrate a microchip in each well to collect rich information from cells, pre-process cell data and streamline it to the paired software. As a result, much more and better data can be extracted, which can be referred to as cell big data. This is fed to an AI with the aim of simplifying interpretation and enabling researchers to discover more information about drugs being tested.
Preclinical studies are vital for early identification of any safety and efficacy issues, and for increasing the chance of success of those drugs entering clinical trials. However, common estimates show that there is still a lot to improve in this area as 90% of the drugs that enter into human trials fail. Among the factors responsible for this high failure rate are the limitations of conducting preclinical tests on animal models. The vast majority of drugs that pass tests on animals do not later work on humans because disease mechanisms vary significantly between species.
New biotechnologies like human stem cells offer more successful alternatives to animal models. These may be in the form of cell culture assays or more sophisticated spheroid and organoid assays that can be used as testbeds for drugs. Human cell-based assays hold the promise to revolutionize drug development, improving the success rate and pushing animal tests towards extinction.
Whatever cell-based assay one wants to investigate, traditional approaches extract cell information via optical systems or a few sensors embedded in plastic substrates. Typically, these substrates are multiwell plates with 24, 96 or even more wells, where each well houses the cell samples to be measured. From these wells, the acquired information travels on limited bandwidth across long distances (compared to the cell size) before reaching a CPU where it gets processed. Long distances and bandwidth issues affect the quality and quantity of information and, consequently, prevent an accurate inspection of cell networks. The CorePlate technology devised by 3Brain disrupts this paradigm by bringing the processing unit in contact with the cells.
“We want to radically change the way preclinical drug screening is performed, offering pharmaceutical companies the opportunity to manage their pipelines more efficiently,” says Mauro Gandolfo, CEO and co-founder of 3Brain AG. “Basically, we take traditional plastic multiwell plates and we turn them into intelligent devices with multi-core processing power, which can be programmed according to research needs. Integrated intelligence is a remarkable distinction from other instruments in the preclinical space. Importantly, despite the large amounts of data CorePlate devices can process, the user can still easily generate results thanks to the AI-driven interpretation of data. With the CorePlate technology, pharmaceutical companies can save time and money by better filtering and prioritizing drug candidates. Our ultimate goal is to provide our users with tools that help them more readily identify new cures for diseases.”
https://clinlabint.com/wp-content/uploads/sites/2/2021/06/3brain.jpg696926panglobalhttps://clinlabint.com/wp-content/uploads/sites/2/2020/06/clinlab-logo.pngpanglobal2021-06-14 10:20:042021-06-14 10:20:043Brain’s CorePlate technology combines microchips and AI in a revolutionary new approach to drug discovery
Sample preparation is important in any type of chromatography analysis. While it can add on extra time, the process of cleaning up samples before injection onto a system results in a range of benefits to the analyst – better recoveries, more reproducible analysis, less downtime of instruments, reduction of troubleshooting, as well as less complex chromatograms due to the reduction of unwanted compounds being injected. All of these can result in time saved which could be needed for repeated work or maintenance on instruments.
Traditional SPE products consist of a loose-filled resin sandwiched between two frits. While this is known to work, it can come with some problems which can complicate analysis or result in poor data being produced. These problems are a result from how the product is packed into a well or cartridge – voiding can occur under the top frit, channels could form through the resin bed which can cause less efficient interactions between the resin and the analyte(s) or there could be variation on compression or resin weight that was dosed into each product.
Figure 1. Common issues associated with loose-filled SPE methods
The Microlute® CP SPE products consist of a unique hybrid design of a solid interconnected network of evenly distributed pores combined with retentive media. The advantage of this design is that flow through the product is consistent and increases the interaction between the analytes and retentive media present within the structure. These two features combined results in a product which offers both high recovery values as well as reproducible results. This technical note uses the 30 mg Microlute® CP Strong Cation Exchange (SCX) 96 well plate to compare performance in recoveries and reproducibility against five competitor loose-filled 30 mg SCX products.
Figure 2. Schematic of the hybrid polymeric structure showing the porous structure of the frit with the active resin immobilised throughout the pore structure
Introduction
The Microlute® CP SCX product is a mixed-mode polymeric SPE product – a combination of ion exchange and reversed phase. This results in a product that has two retention mechanisms which can be fine-tuned to allow more flexibility in the SPE method. The reversed phase functionality allows for separation of analytes on hydrophobic interactions, allowing for retention to be altered by organic modifier concentration. Whereas ion exchange allows for selective strong ionic interactions between the resin and the charged analytes. The introduction of polymeric resins to SPE has resulted in some extra advantages over using silica-based resins [1]. These include:
Polymeric structures do not contain any of the highly active sites found in These include silanol groups which can cause unwanted secondary interactions with analytes which in some cases could cause irreproducible recoveries.
Silica’s structure is also very susceptible outside of the pH range of 2 – 5. If pH is outside this range, hydrolysis of the silica or bonded functional group on the surface could occur. [2] This will result in very poor and irreproducible recoveries. On the other hand, polymeric resins are resistant to pH allowing them to work over the whole pH range (pH 0 – 14).
Functional groups bonded to a silica surface need to be conditioned with an organic solvent to activate the retention mechanism then equilibrated with an aqueous Whilst polymeric resins do not necessarily need to go through this conditioning step.
Polymeric resins are less sensitive to drying out during the SPE process where silica resin can become dry and lose their retentive function. [3]
Strong ion exchange does not work well for strongly acidic or basic analytes (analytes which have a charge over the whole pH range) due to the irreversible binding of charged analyte to the charged ion exchange resin. Weak analytes do work well with strong ion exchange. This is because it is possible to turn the analyte’s charge on or off for a weakly acidic or basic analyte allowing selective binding with a change of pH. To optimise this binding, the 2 pH rule is applied:
When the pH of the solution in which an analyte is dissolved is equal to the pKa value of the analyte, the analyte is 50% ionised, pH can then be used to adjust how ionised a compound is:
Figure 3. A diagram to show the 2 pH rule for a weak acid compound and weak basic compound , with a pKa of 4 and 8, respectively
For a typical strong ion exchange method, analytes are pre-treated to a pH where they are charged and then loaded onto the resin. This allows them to bind strongly to the resin with the ionic interactions. The same pH will be maintained on the wash steps of the method allowing the analyte(s) to keep binding to the resin while washing off interfering com- pounds. To allow elution, pH is changed to neutralise the charge allowing the analyte(s) to stop binding to the resin and elute from the product.
A stock of 1,000 µg/ml of all the basic analytes was made in methanol. A basic load solution was made by diluting 500 µL of the stock solution to 50 mL with water containing 0.1% (v/v) formic acid.
Solid Phase Extraction Method
For both the Microlute® CP SCX product and competitor products, a total of 12 wells were tested. Each well tested was conditioned with 1,000 µL methanol, then equilibrated with 1,000 µL of water. 1,000 µL of basic load solution was then loaded onto the plate in full. Once loaded, 1,000 µL of water containing 0.1% (v/v) formic acid solution in water was used to wash the sorbent. Followed by a strong organic wash of 0.1% (v/v) formic acid solution in methanol.
To elute the analytes of interest, 500 µL of methanol containing 5% (v/v) ammonia was used. The eluent was then evaporated to dryness at 35°C under N2 using a Porvair Sciences Ultravap® Levante (# 500226). A repeat elution was performed using another 500 µL of methanol containing 5% (v/v) ammonia and evaporated to dryness with the same method.
To reconstitute each sample, to each collection vial, 10 µL of 1,000 µg/ml caffeine (ISTD) was added, followed by 790 µL of 60% (v/v) methanol/water containing 0.1% (v/v) formic acid to create a 12.5 µg/mL solution. A further dilution was made using 40 µL of 12.5 µg/mL solution with 760 uL of 60% (v/v) methanol/water containing 0.1% (v/v) formic acid, creating 0.625 µg/mL solutions ready for injection.
Results and Discussion
Figure 4. Chromatogram of basic analytes calibration standard.
Peak assignments can be found in Table 3.
Table 3. Properties and MS parameters for the basic compounds analysed – aPredicted value from Pubchem [4]
Recovery Comparisons against Competitors
Figure 5. Analyte recovery comparisons against equivalent competitor SPE products.
Reproducibility Comparison against Competitors
Figure 6. Analyte %RSD comparisons against equivalent competitor SPE products.
For reproducibility, a lower %RSD means the recovery was more reproducible. The analyte’s %RSD values can be seen in Figure 6. The Microlute® CP SCX managed to maintain a %RSD value of less than 2.6% for every compound analysed. It outperformed every competitor for reproducibility on each compound with only amitriptyline being closely matched. There was no issue of irreproducible results for either the most extreme hydrophilic compound or the most hydrophobic. This again shows that the Microlute® CP SCX product is performing excellently across a wide range of basic analytes.
Summary
The Microlute® CP SCX 30 mg 96 well plate can effectively retain a wide range of hydrophilic and hydrophobic basic compounds. It offers advantageous recoveries across the range of different classes of analytes. Lower %RSD values are seen when comparing against competitor plates for every compound analysed in this study – 1.6%RSD on average for the Microlute® CP SCX compared to the 2.4%RSD (best competitor) and 5.9%RSD (worst competitor). This ensures the product gives reliable and reproducible results which is an important metric in testing where confidence in the data output is required.
References
F. Poole, Solid-Phase Extraction, Elsevier, 2020, p. Chapter 3: Porous polymer sorbents.
V. Brady and J. V. Walther, “Controls on silicate dissolution rates in neutral and basic pH solutions at 25°C,” Geochimica et Cosmochimica Acta, vol. 53, no. 11, pp. 2823-2830, 1989.
N. Qureshi, G. Stecher, C. Huck and G. K. Bonn, “Preparation of polymer based sorbents for solid phase extraction of polyphenolic compounds,” Central European Journal of Chemistry, vol. 9, no. 2, pp. 206-212, 2011.
Copyright 2021. Porvair Sciences Ltd. All rights reserved.
Whilst every effort has been made to ensure the accuracy
of this document, due to continuous product development,
the data contained is subject to constant revision
and Porvair Sciences Ltd. reserves the right to change,
alter or modify its contents. Porvair Sciences and
JG Finneran Associates, Inc., and Kbiosystems
are divisions of Porvair plc.
https://clinlabint.com/wp-content/uploads/sites/2/2021/05/porvair_logo_sq_web.jpg680680panglobalhttps://clinlabint.com/wp-content/uploads/sites/2/2020/06/clinlab-logo.pngpanglobal2021-05-24 10:46:342021-06-01 12:44:50Evaluation of Recovery of Basic Analytes with Microlute® CP 30 mg Mixed-Mode Strong Cation Exchange (SCX) SPE by HPLC-MS
Anion dysregulation is seen in numerous diseases, from cystic fibrosis and chronic pain, to autism and cancer. Researchers at the University of Dallas are investigating anion transport in vivo, developing novel tools to help analyse the effects of both normal and aberrant anion regulation at the molecular level, and are using INTEGRA’s PIPETBOY acu 2 to streamline their cell culture workflows.
Whitney Ong, a joint chemistry graduate student in the Dodani and Smaldone labs at the university, explained: “My research focuses on the development of supramolecular-based sensors for anions, particularly for chloride. The reason we are especially interested in chloride is that it is the most abundant anion in the human body – serving all sorts of functions – and its dysregulation is implicated in diseases such as cystic fibrosis. We’re hoping that our chloride sensing platform could help in its detection, as well as in the diagnosis of a number of other diseases.”
Ong added: “We have three PIPETBOY pipette controllers and use them regularly for a variety of tasks during our cell culture set-up, for instance to make up large volumes of cell culture media, aliquoting and dispensing reagents such as media or antibiotics, resuspensions, as well as to mix different solutions. If we are working with volumes of liquids above 2 ml, we will always use the PIPETBOY; it’s much easier, safer and more efficient that pouring liquids between glass measuring cylinders, where there is a higher risk of spillages or not pouring out the desired volume. They fit into our workflows perfectly – everyone in the lab loves them.”
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