BBI Solutions has launched a new Jo-1 human chimeric antibody for use as a calibrator or positive control in assays to diagnose conditions such as connective tissue disease (CTD).
BBI has used cell culture technologies in response to the concerning situation, as hospital visits continue to be dominated by Covid-related cases, leading to a drop in supplies of positive patient materials for certain markers, which are commonly used for calibrators and controls in IVD assays.
Idiopathic inflammatory myopathies
Autoantibodies against the aminoacyl-tRNA synthetase Jo-1 can be found in up to 20% of patients with idiopathic inflammatory myopathies (IIM), a sub-form of CTD [1]. Jo-1 is therefore the most prevalent marker found in IIM.
Anti-synthetase autoantibodies, such as Jo-1 autoantibodies, are also strongly associated with interstitial lung disease (ILD) which makes an early diagnosis on a component level even more important.
It is the only commercially available Jo-1 human chimeric monoclonal antibody available on the market for companies which manufacture diagnostic tests.
Mario Gualano, CEO of The BBI Group, said: “The introduction of Jo-1 HumAb IgG further strengthens our wide portfolio of CTD products in our autoimmune range. This product will help support IVD companies to realise the growth in the autoimmune diagnostic testing market.”
Human chimeric monoclonal antibodies
Human chimeric monoclonal antibodies can be used as an alternative to characterised disease state plasma. They are produced in transgenic mouse strains in which the sequence for mouse IgG1 Fc region is substituted with the human sequence. After mouse immunisation and use of hybridoma technology, antibodies are generated that retain a human constant region required for recognition by the anti-human IgG detection antibody.
Simon Packer, Product Manager for Antigens at BBI Solutions, explained: “Currently fewer people are visiting hospitals for diseases and conditions other than Covid-19. This is affecting supplies of positive patient materials for certain markers, which are commonly used for calibrators and controls in IVD assays. That is why bringing new chimeric antibodies to the market, at this time, is crucially important to ensure a reliable sustained supply.
“Our Jo-1 HumAb IgG has been developed with the same rigour as our other human chimeric monoclonal antibodies. The mouse monoclonal variable region was carefully defined for Jo-1 and this was integrated with the human constant region in a stabilised cell line. The material is produced using cell culture In-Vitro, meaning there is a continuous steady supply and consistent assay performance, making it a reliable alternative to characterised disease state plasma.
“It has been proven to show equivalent sensitivity to anti-Jo-1 positive patient samples, even at high dilution using our Histidyl-tRNA Synthetase (Jo-1) antigen. So, you can be satisfied that our Jo-1 HumAb IgG works as effectively as characterised disease state plasma.”
Enhanced performance
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, /in Advertenties /by Nova Biomedical Corp.Porvair Sciences expands range of microplate products for SARS-CoV-2 nucleic acid purification
, /in Product News /by panglobalPorvair Sciences has further expanded its range of high-performance microplate products for SARS-CoV-2 nucleic acid purification.
The new KF deep well plate and tip comb plate combo are specifically designed to enhance the performance and productivity of the market-leading Thermo Scientific KingFisher range of nucleic acid purification systems.
Krzysztof Kielmann, microplates product manager at Porvair Sciences commented: “The Kingfisher Flex and Duo Prime systems have a number of design features that make the design of the deep well and the protective tip comb plate crucial to the correct operation of the instrument. Our optimised deep well plate has small gaps that align to locating pins on the Kingfisher instrument and the bottom profile of the 96 wells is designed to fit the heater block providing close contact and sample temperature control.
“The protective polypropylene tip comb is designed specifically for the 96 magnetic probes of the Kingfisher magnetic particle processor. The magnet slides into the disposable 96 well comb dips. Our KF deep well plate together with the protective tip comb plate has been shown to significantly improve the yield and quality of the isolated protein or nucleic acid when used on KingFisher systems.”
The KF range of low affinity deep well plates and protective tip cone plate are manufactured in a cleanroom production environment using ultra-pure polypropylene that has the lowest leachables, extractables and is free from DNase and RNase. This allows SARS-CoV-2 test samples to be purified with the confidence of no risk of contamination or interference during the magnetic particle processing used by KingFisher nucleic acid purification systems.
BBI Solutions markets first chimeric Jo-1 HumAb IgG
, /in Product News /by panglobalBBI Solutions has launched a new Jo-1 human chimeric antibody for use as a calibrator or positive control in assays to diagnose conditions such as connective tissue disease (CTD).
BBI has used cell culture technologies in response to the concerning situation, as hospital visits continue to be dominated by Covid-related cases, leading to a drop in supplies of positive patient materials for certain markers, which are commonly used for calibrators and controls in IVD assays.
Idiopathic inflammatory myopathies
Autoantibodies against the aminoacyl-tRNA synthetase Jo-1 can be found in up to 20% of patients with idiopathic inflammatory myopathies (IIM), a sub-form of CTD [1]. Jo-1 is therefore the most prevalent marker found in IIM.
Anti-synthetase autoantibodies, such as Jo-1 autoantibodies, are also strongly associated with interstitial lung disease (ILD) which makes an early diagnosis on a component level even more important.
It is the only commercially available Jo-1 human chimeric monoclonal antibody available on the market for companies which manufacture diagnostic tests.
Mario Gualano, CEO of The BBI Group, said: “The introduction of Jo-1 HumAb IgG further strengthens our wide portfolio of CTD products in our autoimmune range. This product will help support IVD companies to realise the growth in the autoimmune diagnostic testing market.”
Human chimeric monoclonal antibodies
Human chimeric monoclonal antibodies can be used as an alternative to characterised disease state plasma. They are produced in transgenic mouse strains in which the sequence for mouse IgG1 Fc region is substituted with the human sequence. After mouse immunisation and use of hybridoma technology, antibodies are generated that retain a human constant region required for recognition by the anti-human IgG detection antibody.
Simon Packer, Product Manager for Antigens at BBI Solutions, explained: “Currently fewer people are visiting hospitals for diseases and conditions other than Covid-19. This is affecting supplies of positive patient materials for certain markers, which are commonly used for calibrators and controls in IVD assays. That is why bringing new chimeric antibodies to the market, at this time, is crucially important to ensure a reliable sustained supply.
“Our Jo-1 HumAb IgG has been developed with the same rigour as our other human chimeric monoclonal antibodies. The mouse monoclonal variable region was carefully defined for Jo-1 and this was integrated with the human constant region in a stabilised cell line. The material is produced using cell culture In-Vitro, meaning there is a continuous steady supply and consistent assay performance, making it a reliable alternative to characterised disease state plasma.
“It has been proven to show equivalent sensitivity to anti-Jo-1 positive patient samples, even at high dilution using our Histidyl-tRNA Synthetase (Jo-1) antigen. So, you can be satisfied that our Jo-1 HumAb IgG works as effectively as characterised disease state plasma.”