KPL offers next-generation solutions to improve sensitivity and time to results in Western blotting applications. New SignaLOCK™ HRP ChemiWestern Kit (Imager), SignaLOCK HRP ChemiWestern Kit (Film), and SignaLOCK AP ChemiWestern Kit (Film/Imager) enable researchers to reach the highest levels of detection sensitivity. These kits enable detection in the nanogram range with only a 1-minute exposure. A longer exposure offers sensitivity in the femtogram range. The high sensitivity of SignaLOCK kits allows you to use primary and secondary antibodies at higher dilutions, minimizing background and lowering cost. They combine a chemiluminescent substrate with a unique non-protein blocker and wash solution in one powerful system. Whether you prefer peroxidase or phosphatase detection, film or imager analysis, SignaLOCK kits enable researchers to see more signal with less background in their Western blots. All kits offer comprehensive protocols and can be used with both nitrocellulose and PVDF membrane.
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Lab automation can help handle increasing workloads from the growing demand for in vitro diagnostic testing and allows better use of personnel. The circular track and modular design of the Aptio Automation platform ensures that it can be adapted to nearly any lab or testing environment. It also offers connectivity to Siemens’ portfolio of automation-ready analysers, thus facilitating a multidisciplinary approach to testing. The point-inspace aspiration feature helps streamline workflow by reducing the need to aliquot (or divide) samples, and its puck-based system with radio frequency identification enables individual sample routing and tracking, along with STAT prioritization. The system delivers comprehensive analytics via powerful, centralized information technology, while having the flexibility to provide pre- and post-analytical capabilities. The company’s CentraLink data management system facilitates efficient sample flow, auto-verification, quick access to samples and proactive quality control. This can also be customized for the end user, which speeds up system access and supports error reduction.
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With 360° rotation, the 6-position pipette work station, TwisterTM universal 336, enables quick and easy instrument selection. Storage space is provided for six pipettes of most brands, and the interchangeable discs are available in a choice of seven translucent colours. The unit is simple to disassemble and reassemble for cleaning.
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The OneWell Plate is a cell culture dish with a compartment that is particularly suitable for cultivating and analysing bacteria. Its automation-friendly plate format enables automated replication of bacteria colonies. The rectangular, stackable plate allows highly efficient use of incubator space. A model with a surface hydrophilized by physical treatment is available for adherent cell culture. The FourWell Plate, with four individual chambers, offers space for four slides, thereby enabling parallel experiments. Cell and tissue samples cultivated on these slides can be supplied with fresh medium quickly and easily and examined directly under a microscope. Semi-circular cavities in the base of each well facilitate the removal of slides with tweezers, and spacers prevent the slides from slipping to the edge of the well and covering these cavities. Both plates, made of transparent polystyrene, are sterile and free of detectable pyrogens, human DNA and DNases/ RNases. Their external dimensions comply with ANSI microplate standards and the plates can be used with a variety of automated systems. A bevelled edge on lid and dish ensures the lid can only be applied in one direction. The plates can also be used as multi-purpose containers for washing membranes (Southern, Northern and Western blots), for example.
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Delivering increased sensitivity, the Triple Quad 6500 and QTRAP 6500 systems are next-generation mass spectrometers designed for those who need to push the limits of quantitation in complex matrices while maintaining reproducibility and robustness. With new multi-component Ion- Drive technology increasing the generation, transmission and detection of more ions, the instrument delivers sensitivity enhancements with no compromise in mass range. This is made possible by significant improvements in the ionization source through the new Turbo V source, enhancements in the ion transmission stage with the novel QJet guide, and a 20x increase to the detector dynamic range with the High Energy detector. The 6500 platform is also compatible with the novel SelexION Technology, which delivers a new dimension of selectivity in differential ion mobility spectrometry for quantitative and qualitative analysis. The 6500 series is ideally suited to a variety of applications, including drug discovery and development in regulated laboratories; peptide quantitation as well as biomarker verification in biomedical research; and clinical research in endocrinology laboratories.
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