Thermo Fisher Scientific unveils spectralunmixing beads for enhanced flow cytometry precision
Thermo Fisher Scientific has introduced Invitrogen UltraComp eBeads Spectral Unmixing Beads, a next-generation com-pensation tool designed to improve signal accuracy in spectral and conventional flow cytometry applications. The product addresses persistent challenges in fluoro-chrome separation and background inter-ference that can compromise data quality in multi-parameter cellular analysis.
Addressing fluorescence signal overlap
Flow cytometry researchers analysing complex cellular phenotypes increasingly require high-parameter approaches to characterise rare cell populations and track multiple markers simultaneously. However, overlapping fluorescent signals and auto-fluorescence background can generate false positives and negatives, limiting analytical precision. The UltraComp eBeads system is engineered to behave similarly to authentic cells whilst reducing autofluorescence and enhancing fluorochrome signal clarity.
“With this launch, scientists now have access to advanced compensation beads that perform like real cells and are compatible with the existing line of spectral and conventional flow cytometers in the market, including our recently introduced Attune Xenith flow cytometer,” said Trisha Dowling, Vice President/General Manager, Flow and Imaging Technologies. “With distinct separation of fluorochrome signals and fewer errors in flow cytometry data analysis, researchers will be able to confidently detect and quantify cell populations in their studies.”
Broad compatibility and simplified workflows
The compensation beads offer multispecies antibody compatibility, binding with mouse, rat, hamster, rabbit, and recombinant human antibodies – representing the broadest species support currently available. The system supports all major laser lines, including UV, violet, blue, green, yellow-green, red, and infrared wavelengths, enabling integration with diverse cytometer configurations. Each preparation contains both positive and negative bead populations within a single drop, creating distinct dual peaks that simplify setup procedures, particularly for high-parameter panels requiring numerous fluorochrome channels.
Target applications
The product is designed for research use by immunologists and immuno-oncologists in academic institutions, pharmaceutical and biotechnology companies, and flow cytometry core facilities. The technology aims to support investigations into disease mechanisms and therapeutic target discovery by providing more comprehensive cellular profiling with reduced technical variability across experiments.
The UltraComp eBeads deliver unmixing and compensation performance comparable to authentic cellular samples whilst maintaining bright, consistent fluorophore signals and minimising fluorescence background noise. This combination is intended to yield more reliable downstream results with cleaner spectral signatures for complex multicolour panels.
Thermo Fisher Scientific’s Invitrogen UltraComp eBeads Spectral Unmixing Beads are for research use only, not for use in diagnostic procedures.
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