Biotium launches GlycoLiner kits for cell surface labelling
Biotium has announced the launch of GlycoLiner™ Cell Surface Glycoprotein Labelling Kits, offering researchers a rapid method for selective labelling of cell surface glycoproteins. The kits employ next-generation aminooxy chemistry that enables covalent labelling under gentle conditions.
Fast labelling at neutral pH
The GlycoLiner system distinguishes itself from conventional aminooxy reagents through its self-catalysing chemistry, which functions efficiently at neutral pH without requiring harsh buffers, additional catalysts, or high label concentrations. Surface labelling can be completed in approximately 20 minutes at room temperature or 4°C, supporting workflows that require low-temperature conditions to prevent protein internalisation.
The kits provide options for biotin labelling or a selection of five bright fluorescent dyes, enabling researchers to choose labels suited to their specific applications.
Compatibility with downstream analysis
GlycoLiner labelling is covalent and compatible with common fixation and permeabilisation protocols. This compatibility extends to protein extraction for capture of biotinylated glycoproteins, enabling downstream immunofluorescence staining, microscopy, flow cytometry, and biochemical analysis.
A notable feature of the system is its reduced intracellular staining in dead cells, which enables clearer visualisation of cell boundaries compared to other covalent cell surface stains that can produce strong labelling of dead cell cytoplasm.
Expanding membrane labelling options
“Biotium has launched an extensive selection of unique membrane-labelling probes that support different workflows. We are excited to provide yet another option that offers highly selective imaging of live cell surface glycoproteins that is also compatible with fixation,” said Lori Roberts, PhD, Director of Bioscience.
The GlycoLiner technology adds to Biotium’s portfolio of cell staining technologies, which includes options for staining fixed cells and long-term imaging in live cells. The system is designed to support cell surface biology research with applications in multiple areas of life science investigation.
Key specifications
The kits offer several features for laboratory workflows:
• Complete labelling protocol in approximately 20 minutes
• Operation at neutral pH without catalyst requirements
• Choice of five fluorescent dyes spanning blue to near-infrared wavelengths, plus biotin
• Highly selective live-cell surface labelling with reduced dead-cell background
• Compatibility with fixation, permeabilisation, and cell lysis protocols
GlycoLiner Kits are available through Biotium and authorised distributors worldwide.
For more information, visit: https://shorturl.at/Aj1wE
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