Biotium launches ViaTag haloalkane ligands for HaloTag fusion protein labelling
Biotium, a Fremont, California-based provider of fluorescent reagents and kits for life science research, has announced the release of ViaTag™ Haloalkane Ligands, a new line of fluorescent ligands designed for labelling HaloTag® self-labelling protein tags in live or fixed cells.
Broad spectral range and dual-format availability
Available in 11 fluorescent colours spanning blue to near-infrared, the ViaTag ligands are designed to support multicolour imaging and flow cytometry applications. The products are offered in both cell membrane-permeant and impermeant formats, enabling researchers to target either intracellular proteins or cell-surface tagged proteins according to experimental requirements.
HaloTag is a self-labelling protein tag that forms a covalent bond with fluorescent haloalkane ligands, providing stable, fluorescence-based detection. According to Biotium, ViaTag ligands “deliver bright, durable labelling that remains robust through fixation, permeabilisation, and protein extraction,” making them suitable for use across a range of sample preparation protocols common in clinical and research laboratory settings.
Streamlined protocols without extensive wash steps
A notable feature of the ViaTag range is the elimination of the extensive wash steps typically associated with haloalkane ligand labelling. The ligands are supplied as stable, ready-to-use DMSO stock solutions, which the company states enable “fast labelling with bright signal and low background.” This is presented as a significant workflow advantage over comparable products currently on the market, with Biotium positioning ViaTag as providing “streamlined labelling protocols and lower overall cost.”
Applications and portfolio context
The ViaTag range is compatible with both live and fixed cell preparations, and its resistance to standard processing steps – including permeabilisation and protein extraction – broadens its utility for laboratory scientists working across diverse experimental protocols.
Biotium notes that ViaTag Haloalkane
Ligands “expand the company’s portfolio of high-performance fluorescent labelling tools, offering researchers a more economical, efficient, and versatile solution for HaloTag-based imaging and detection across
diverse experimental workflows.”
The products complement Biotium’s existing cellular staining portfolio, which includes NucSpot® Live Cell Nuclear Stains and CellBrite® Steady Membrane Staining Kits for long-term imaging of nuclei and membranes in live cells.
For more information, visit: https://biotium.com/product/viatag-haloalkane-ligands
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