Leptin human ELISA
Leptin, the product of the ob (obese) gene, is a single-chain 16 kDa protein consisting of 146 amino acid residues. Leptin is produced mainly in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and body weight regulation. It targets the central nervous system, particularly the hypothalamus, affecting food intake. The primary effect of leptin appears to be mediated by leptin receptors expressed mainly in the hypothalamus. In humans, leptin levels correlate with body mass index (BMI) and percentage body fat, and are elevated even in obese individuals. Leptin has a dual action; it decreases the appetite and increases energy consumption, causing more fat to be burned. Leptin is secreted in circadian fashion with nocturnal rise in both lean and obese patients. Mutations of the ob gene resulting in leptin deficiency are the cause of obesity in the ob/ob mice. Endogeneous leptin can normalize their body weight. In contrast, high levels of leptin in obese human subjects point to an insensitivity to endogeneous leptin. Other factors in addition to the amount of body fat appear to regulate leptin action: insulin, glucocorticoids, catecholamines and sex hormones. Studies have shown that leptin may be linked to reproductive function.
The BioVendor Leptin ELISA is CE-marked, allowing in vitro diagnostic use in the European Union (research use only in the rest of the world). This sandwich ELISA uses a HRP-labelled antibody and has a total assay time of less than 2.5 hours. The kit measures total leptin in a 96-well assay format. Quality controls are human serum-based and standards are recombinant protein-based. Components of the kit are provided ready to use, concentrated or lyophilized.
Supplier: BioVendor Research and Diagnostic Products
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