Bordetella pertussis test kit
Pertussis toxin is a protein exclusively produced by the bacterium Bordetella pertussis, which causes the respiratory infectious disease pertussis (whooping cough) in humans. The typical symptoms are frequent and severe cough attacks with a whooping sound and vomiting. The infection can be fatal for infants that have not yet been vaccinated against pertussis. Vaccination does not provide lifelong protection. Adult pertussis can be difficult to diagnose due to milder symptoms and often due to the lack of the characteristic whooping sound and vomiting. Adults with pertussis are therefore the main source of infection for unvaccinated infants. Diagnosis and treatment of adult pertussis can eventually reduce infant pertussis cases. Bordetella pertussis is only present in the airways for the first few weeks after the infection but the symptoms last for a longer period due to presence of the toxin and several other virulence factors. Diagnostic methods detecting the bacterium itself (culture and PCR) are therefore only useful early in the infection. Serology is, however, very efficient for diagnosis of patients with prolonged cough. Pertussis toxin is the recommended antigen for pertussis ELISA as there is no risk of cross-reactions with other bacterial antigens. The Bordetella pertussis IgG-PT ELISA kit is a traditional ELISA setup, which has been developed using purified pertussis toxin. Four standard samples are provided to establish the standard curve. The dilution method is the same for the standards and samples. Heat-treated sera can be analysed with this method without the risk of false-positive results. The sensitivity and specificity value is 81 % and 96 % respectively based on data from Denmark using a cut-off at 75 IU/mL. The test can be carried out either in one day or with overnight incubation for the first step. The plate can be split to facilitate more than one run of the kit. The kit contains enough reagents including controls to perform 24 – 44 test. Shelf life is minimum one year from date of shipment.
Supplier: SSI Diagnostica A/S
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