Amsbio has announced StemFit™ Basic05, an animal origin-free medium designed for scalable human pluripotent stem cell (hPSC) manufacturing. Compatible with both 2D adherent and 3D suspension culture, the medium enables a workflow from cell expansion through to process scale-up using a single medium.

One medium across culture modes

According to the company, using one medium across culture modes minimises the need for the process re-development and optimisation traditionally associated with media changes. Pluripotent stem cells cultured in StemFit Basic05 also retain their differentiation capability, and have been confirmed as capable of differentiation directly from 3D aggregates.

Viability and spheroid formation

For laboratories seeking to implement stable and scalable suspension culture, high cell viability and uniform spheroid formation are vital prerequisites. Amsbio says StemFit Basic05 has been demonstrated to deliver high cell viability and uniform spheroid formation reliably across multiple human iPSC lines, along with maintenance of a normal karyotype. The company describes the spheroid formation as robust and highly reproducible, and says the medium reduces process complexity and development burden.

Flexible culture scheduling

StemFit Basic05 supports flexible culture scheduling, with comparable cell expansion reported even when weekend feeding was omitted.

Amsbio, part of the Europa Biosite group, supports drug discovery, translational research, and cell and gene therapy across Europe, North America and wider international markets. Its integrated stem cell platform combines StemFit media, iMatrix™ recombinant laminins and CELLBANKER™ cryopreservation technology.

For more information, visit: https://www.amsbio.com/stemfit-stem-cell-culture/

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