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Biotium expands selection of novel nuclear stains for enhanced multiplexing flexibility for microscopy and flow cytometry

Biotium, a leader in fluorescent dyes for life science research, has expanded its NucSpot Nuclear Stains line-up, offering unprecedented specificity and flexibility for nuclear counterstaining.

NucSpot Nuclear Stains are membrane-impermeant, targeting the nucleus selectively with minimal fluorescence until binding to DNA, enabling convenient no-wash nuclear staining. Unlike traditional options such as propidium iodide (PI), TO-PRO, or TOTO, NucSpot achieves nuclear specificity in fixed and permeabilized cells without the need for RNase treatment. With an extensive selection of 9 colours spanning the fluorescence spectrum from green to near-infrared, NucSpot Nuclear Stains provide greater panel flexibility, compatible with FITC, Cy3, PE, Texas Red, Cy5, APC, Cy5.5, and Cy7 channels. Several options are non-toxic, suitable for cell cycle profiling by flow cytometry, and can be incubated in live cultures for multi-day imaging.

“These new additions to our cellular stains line provide a major improvement by broadly expanding scientists’ colour choice for nuclear counterstaining. Plus, NucSpot Nuclear Stains are much more photostable than existing cyanine-based nuclear stains, and there’s no need to treat with RNase for nuclear staining,” said Dr Lori Roberts, Director of Bioscience at Biotium. “These novel dyes are products of our commitment to providing cutting-edge and convenient tools for cell biology research.”

Features and benefits of NucSpot Nuclear Stains
• Bright & Specific: Selective nuclear counterstains with exceptional sensitivity.
• Versatility: Suitable for fixed cells or dead cell staining in live cultures.
• User-Friendly: No-wash, no RNase staining protocol for efficient and consistent results.
• Exceptional Photostability: Maintains excellent imaging quality during prolonged sessions.
• Wide Colour Selection: Available in 9 colours for flexible panel design.
• Validation and Quality Assurance: Rigorous in-house testing guarantees reliability and consistency.

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PFA-fixed, Triton X-100 permeabilized HeLa cells stained with NucSpot Nuclear Stains in PBS without a wash step. All stains were used at 1X concentration except for NucSpot 750/780, which was used at 5X concentration to image using 640 nm excitation.