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Biotium introduces novel antibody labelling kits for rapid labelling of IgM antibodies

Biotium has released Mix-n-Stain CF Dye IgM antibody labelling kits. These kits revolutionize the labelling process for IgM antibodies, enabling researchers to easily obtain high-performance fluorescent IgM conjugates with minimal hands-on time with no purification after labelling.

IgM antibodies offer advantages over IgG anti-bodies due to their increased avidity. However, traditional methods for conjugation of IgG anti-bodies are frequently ineffective for optimal labelling of IgM antibodies due to differences in the immunoglobulin structures. Recognizing this roadblock, Biotium has successfully opti-mized the reaction conditions and developed these kits to address this issue.

Biotium IgM Mix n Stain tubulin composite

Mouse anti-beta tubulin clone 5H1 (IgM kappa) was labeled with CF®488A using Mix-n-Stain™ IgM labeling, and then was used to stain methanol-fixed HeLa cells at 5 ug/mL (right panel). The conjugate shows comparable staining specificity compared
to unlabeled antibody at 1 ug/mL detected using CF®488A Goat Anti-Mouse IgM Mu chain secondary antibody at 2 ug/mL (left panel).

With these kits, researchers can now label 25 ug or 100 ug of their IgM antibody in as little as 15-30 minutes, ensuring rapid experimental workflows. These kits offer unparalleled convenience, with less than 30 seconds of hands-on time required.

Mix-n-Stain CF Dye IgM antibody labelling kits are available in a selection of 8 CF dyes and FITC. CF dyes are Biotium’s next-generation fluorescent dyes designed to provide superior brightness and photostability compared to other commercially available fluorescent dyes.

Features and benefits

• Optimized for IgM: Developed to offer high-performance IgM conjugates
• Quick and easy: Conjugate in as little as 15-30 minutes, no purification required
• Wide selection: Available in your choice of 8 bright CF dye colours and FITC

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Dr Lori Roberts, Director of Bioscience at Biotium, said: “This technology provides a new and simple solution to the longstanding challenge of IgM conjugation. We believe these kits will provide researchers with more flexibility in panel design for immunofluorescence and flow cytometry.”

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