Biotium releases optimized antibody labelling kits for STORM super-resolution microscopy
Biotium, a supplier of cutting-edge life science reagents, is expanding their line of industry-leading Mix-n-Stain labelling kits with new kits designed specifically for producing antibody conjugates ideal for super-resolution stochastic optical reconstruction microscopy (STORM). Mix-n-Stain STORM CF Dye Antibody Labeling Kits are available with Biotium’s unique STORM CF Dyes, which feature exceptional brightness and photochemical properties ideal for STORM imaging. In addition, the kits are optimized to yield a low degree of labelling that is optimal for single molecule localization microscopy techniques such as STORM.
STORM image quality is highly dependent on the properties of both the fluorophore and antibody conjugate. Specifically, antibodies conjugated to bright fluorophores with robust photo-switching properties at a low degree of labelling are ideal for STORM. However, a convenient method to produce antibody conjugates with a low degree of labelling for STORM is lacking. Moreover, while several red-excited dyes suitable for STORM are available (e.g., AF647, CF647, CF660C, and CF680), there are limited options for green-excited fluorescent probes for multi- colour STORM. With the release of Mix-n-Stain STORM CF Dye Antibody Labeling Kits, Biotium has met this challenge by offering a streamlined solution for producing antibody conjugates optimal for STORM imaging in a variety of colours. Mix-n-Stain STORM CF Dye Antibody Labeling Kits are available with 8 STORM-validated CF Dyes with emission ranging from visible red to near-IR. CF583R and CF597R are novel green- excited CF Dyes for multi-colour STORM that were recently published in collaboration with
UC Berkeley Professor and STORM scientist Dr. Ke Xu, PhD.
Each Mix-n-Stain STORM CF Dye Antibody Labeling Kit is suitable for labelling exactly 50 ug antibody. Labelling is rapid and efficient, requiring only 30 minutes of total reaction time with no downstream purification. Mix-n-Stain STORM CF Dye Antibody Labeling Kit features:
- Validated: Choice of 8 CF Dye colors developed and published for STORM
- Green-excitable options: New CF583R and CF597R best-in class green excited STORM dyes
- Low DOL: Labeling yields DOL of 1-2.5, optimal for STORM
- Efficient: Requires only 30-minute total reaction time with no purification after labelling