The EUROArray PneuVir from EUROIMMUN provides multiplex PCR-based detection and differentiation of 17 viruses that cause respiratory diseases in one test. The parameters encompass SARS-CoV-2, influenza virus types A and B, respiratory syncytial viruses, parainfluenza virus types 1 to 4, further human coronaviruses (229E, NL63, HKU-1 and OC43), metapneumovirus, adenoviruses, entero- and rhinoviruses, bocaviruses and parechoviruses. The test is performed on human nasopharyngeal swabs.
In the simple EUROArray procedure, viral RNA isolated from the patient samples is transcribed into DNA by reverse transcription. Pathogenspecific sequences are then amplified and fluorescently labelled in one multiplex polymerase chain reaction (PCR). The PCR products are hybridised to BIOCHIP microarray slides containing immobilised complementary oligonucleotide probes and detected by their fluorescence signals. The evaluation, interpretation and archiving of results is fully automated by EUROArrayScan software and is thus highly standardised and objective. Highly specific primers, ready-to-use PCR components and integrated controls all contribute to the reliability of the analysis. The entire EUROArray process from sample to report release is IVD validated and CE registered.
The test supports diagnosis and differentiation of respiratory infections that are widespread during the winter season. Moreover, simultaneous infections with multiple viruses can be reliably identified. Establishing the causative agent of respiratory diseases can aid targeted treatment and indicate the need to isolate patients where applicable. Detection of viral pathogens can also help to exclude a bacterial infection and thus avoid unnecessary antibiotic treatment.