How are EUROLINE profiles used in autoimmune diagnostics?
Autoimmune diagnostics often require analysis of a large repertoire of autoantibodies, for which EUROLINE is ideally suited. For example, our EUROLINE ANA Profiles enable specific confirmation of up to 23 autoantibodies that give rise to nuclear patterns in indirect immunofluorescence screening on HEp-2 cells. We are proud to say that they are among the top tests used by participants in quality assurance schemes such as INSTAND. Further EUROLINE profiles provide targeted autoantibody detection in myositis, systemic sclerosis, paraneoplastic neurological syndromes, autoimmune liver diseases and coeliac disease.
Our EUROLINE Myositis Profiles offer the largest antigen spectrum available in the line blot format for detection of myositis-relevant autoantibodies, encompassing 20 parameters including the exclusive cytosolic 5′-nucleotidase 1A (cN1A) antigen. The importance of wide antibody testing in idiopathic inflammatory myopathies is highlighted by a case story we featured recently on our blog (https://www.euroimmunblog.com/a-life-saving-diagnosis-thanks-to-comprehensive-antibody-testing-a-motivating-success-story/). It describes a patient who was negative for all the classic autoantibodies and was initially diagnosed with idiopathic lung fibrosis. Follow-up testing with the EUROLINE revealed the presence of the rare anti-signal recognition particle (SRP) and anti-RNA polymerase III (RP11) autoantibodies, enabling a diagnosis of myositis to be secured and life-improving treatment to be started.
What about allergies?
EUROLINE technology is also ideal for broad-range allergy diagnostics, as up to 54 allergens can be analysed on a single test strip. Our EUROLINE profiles are tailored to particular indications, for example, food, inhalation, atopy, insect venoms or pediatrics, or to specific regions. The product range includes more than 65 profiles for multiparameter detection of specific IgE antibodies. Our molecular allergy diagnostic (DPA-Dx) profiles enable precise identification of allergy-triggering components. The importance of different allergen components in risk assessment and immunotherapy decision-making is described in many scientific publications and allergy diagnostic guidelines.
Which immunoblots are available for infection diagnostics?
We offer immunoblots for detection of antibodies against Borrelia, Treponema pallidum, Epstein-Barr virus (EBV), hepatitis E virus (HEV), parvovirus B19 and TORCH pregnancy-relevant pathogens, to name just a few. Our Anti-Borrelia EUROLINE tests are worth a particular mention, as they provide exclusive combinations of early- and late-phase markers, including the major antigens variable major protein-like sequence expressed (VlsE) and outer surface protein C (OspC) from different Borrelia species, together with designer antigens and immunogenic lipids. With our new EUROMicroblot technology, the efficiency of Lyme disease diagnostics can now be increased even further.
Tell us about the new EUROMicroblot
We are excited to introduce our new EUROMicroblot system, which meets our customers’ needs for higher throughput blot analyses. It combines the best of two technologies, providing 96-well microplates fitted with miniaturized line blot strips. The microplates can be processed easily on ELISA instruments such as our EUROLabWorkstation ELISA or EUROIMMUN Analyzer I or 2-P. The technology helps to improve efficiency in labs, as free capacity on ELISA instruments can be used optimally, and screening and confirmatory tests can be processed in one run. The break-off wells of the microplates can be separated individually, so that the number of analyses can be adapted to the lab’s actual needs. All samples, plates and reagents are tracked by barcodes, ensuring traceability throughout the analysis. EUROMicroblot images are subsequently acquired using the compact tabletop Microwell Imager and the results are evaluated using our established EUROLineScan software.