Biotium has released Mix-n-Stain CF Dye IgM antibody labelling kits. These kits revolutionize the labelling process for IgM antibodies, enabling researchers to easily obtain high-performance fluorescent IgM conjugates with minimal hands-on time with no purification after labelling.

IgM antibodies offer advantages over IgG anti-bodies due to their increased avidity. However, traditional methods for conjugation of IgG anti-bodies are frequently ineffective for optimal labelling of IgM antibodies due to differences in the immunoglobulin structures. Recognizing this roadblock, Biotium has successfully opti-mized the reaction conditions and developed these kits to address this issue.

Mouse anti-beta tubulin clone 5H1 (IgM kappa) was labeled with CF®488A using Mix-n-Stain™ IgM labeling, and then was used to stain methanol-fixed HeLa cells at 5 ug/mL (right panel). The conjugate shows comparable staining specificity compared
to unlabeled antibody at 1 ug/mL detected using CF®488A Goat Anti-Mouse IgM Mu chain secondary antibody at 2 ug/mL (left panel).

With these kits, researchers can now label 25 ug or 100 ug of their IgM antibody in as little as 15-30 minutes, ensuring rapid experimental workflows. These kits offer unparalleled convenience, with less than 30 seconds of hands-on time required.

Mix-n-Stain CF Dye IgM antibody labelling kits are available in a selection of 8 CF dyes and FITC. CF dyes are Biotium’s next-generation fluorescent dyes designed to provide superior brightness and photostability compared to other commercially available fluorescent dyes.

Features and benefits

• Optimized for IgM: Developed to offer high-performance IgM conjugates
• Quick and easy: Conjugate in as little as 15-30 minutes, no purification required
• Wide selection: Available in your choice of 8 bright CF dye colours and FITC

Digital issue: Please click here for more information

Dr Lori Roberts, Director of Bioscience at Biotium, said: “This technology provides a new and simple solution to the longstanding challenge of IgM conjugation. We believe these kits will provide researchers with more flexibility in panel design for immunofluorescence and flow cytometry.”

For more information, visit: https://biotium.com/product/mix-n-stain-cf-dye-igm-antibody-labeling-kits