SynGenSys expands CHO.SET promoter library for biomanufacturing
SynGenSys, a biotechnology company designing synthetic gene promoter systems, has introduced CHO.SET® 2.0, an expanded library of synthetic promoters intended to improve protein production in Chinese Hamster Ovary (CHO) cells.
Addressing limitations of natural promoters
The CHO.SET Library® was first introduced in 2024 and has since been expanded and optimised. Current market vectors rely heavily on natural promoters such as CMV and SV40E, which have restricted transcriptional activity and are considered insufficient for generating the high productivities required for modern commercial biotechnology applications.
The synthetic promoters were developed de novo using the company’s proprietary transcriptional analysis and sequence engineering platform, combining computational design with empirical validation to produce a diverse promoter library applicable across a range of CHO expression systems and biomanufacturing requirements.
Higher titres and improved clone selection
Supporting data indicate that CHO.SET promoters outperform standard industry promoters, delivering enhanced transcriptional activity and improved protein production metrics. Selected vectors have achieved over four times higher antibody productivity, with titres exceeding 2 g/L in a simple fed-batch flask model.
The synthetic promoter pools also contain a higher proportion of high-expressing cells, increasing the likelihood of isolating top-performing clones. CHO.SET supports more streamlined cell line development workflows, achieving high titres without the need for MSX, reducing process complexity and enabling faster recovery timelines and route to materials for toxicity studies.
Dr Andrew Racher, Co-CEO of SynGenSys, commented: “CHO cells are the pharmaceutical industry’s gold-standard cell factories, and CHO cell-specific synthetic promoters provide an elegant solution to overcoming key productivity limitations in the industry. We are excited to release this latest update and to continue providing solutions that address evolving biomanufacturing needs.”
Upcoming showcase at Biotech Week Boston
The CHO.SET Promoter Library will be showcased at BioProcess International from 22-25 September, as part of Biotech Week Boston.
For more information, visit: https://www.syngensys.com/biomanufacturing
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