The second in its series of steroid hormone kits based on tandem mass spectrometry, the AbsoluteIDQ Stero17 kit can analyse up to 80 samples per batch, requires a sample volume of only 500 μL, and enables the most comprehensive simultaneous quantification of a steroid panel using HPLC-MS/ MS as well as improved high-throughput UHPLC–MS/MS analysis. The kit covers the standardized quantitative analysis of 17 of the most important steroid hormones from only a single blood sample using UHPLC-MS/MS, a technology meeting the most stringent quality control criteria for routine diagnostics, ensuring a high level of analytical sensitivity and selectivity, and guaranteeing superior accuracy and excellent precision for robust results. The kit, which has been approved by proficiency tests, comes complete with isotopelabelled internal standards, reagents and protocols for sample pre¬paration based on solid-phase extraction in a 96-well plate format and subsequent UHPLC-ESI-MS/ MS analysis in highly specific multiple reaction monitoring (MRM) mode and a tightly quality-controlled workflow. Given the significant role of steroid hormones in the body, their quantitative analysis in clinical diagnostics and research-based applications can be crucial. Compared to immunoassay-based tests, LC-MS/MS analysis offers the advantages of multiplexing (simultaneous analysis of multiple target analytes), higher specificity (by so-called multiple reaction monitoring (MRM), greater dynamic range of the calibration and lower inter-laboratory variability (including diversity in sample preparation, calibrators and analytical methods).
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Immunochromatographic test for T. evansi-specific antibodies
, /in Product News /by 3wmediaInfection with Trypanosoma evansi causes the disease ‘surra’ that affects a wide variety of wild and domesticated animals in India, Africa, Asia, and Latin America, and outbreaks also occur sporadically in Europe. Pyrexia and progressive anemia are major clinical signs of surra, and the principal host species are camels, horses, buffaloes and cattle. Detection of antibodies specific to T. evansi is possible with immunofluorescence (IFA), enzyme linked immunosorbent assay (ELISA) and a card agglutination test (CATT/T.evansi). In collaboration with the Institute of Tropical Medicine (Antwerp, Belgium), Coris BioConcept has developed an immunochromatography test (ICT) for detecting antibodies specific for T. evansi. First validation performed on 88 positives and 73 negatives shows a negative predictive value (NPV) of 100 %. The test is performed in 15 minutes on whole blood or plasma. It is the first ICT test for surra that is intended to be used either in the laboratory or in the field.
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Fast clean-up of plasma samples with phospholipid removal plates
, /in Product News /by 3wmediaSuccessful chromatographic analysis of plasma samples requires the removal of both proteins and phospholipids, which can clog HPLC/UHPLC columns. Phospholipids can also cause ion suppression and, over time, will reduce sensitivity due to a build-up on the mass spec source. For the fast clean-up of plasma samples in pharmaceutical and clinical research laboratories, Phree phospholipid removal plates remove both proteins and phospholipids and deliver the prepared plasma to a collection plate in one step. The high-capacity sorbent can process up to 400 μL per well and removes 99.0 to 100% of lysophosphatidyl and phosphatidyl cholines, which are not removed when a simple protein precipitation is performed. The one-step clean-up eliminates time-consuming method development, and the plate format enables the simultaneous processing of 96 samples, contributing to significant time savings.
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Reliable, automated nucleic acid extraction
, /in Product News /by 3wmediaProviding high lot-to-lot consistency and quality the MagNA Pure 96 System for automated sample preparation is able to purify a broad spectrum of human samples and offers LIMS (laboratory information management system) connectivity for safer and more convenient data transfer. It provides ready-to-use reagents and uses a minimal number of consumables for purification of nucleic acids from up to 96 clinical samples in less than 60 minutes. The instrument performance characteristics of MagNA Pure have been established in external trials with model parameters. This platform is ideal for clinical trials, medical research and molecular diagnostic testing that requires consistent high-quality sample analysis.
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Accurate steroid hormone analysis
, /in Product News /by 3wmediaThe second in its series of steroid hormone kits based on tandem mass spectrometry, the AbsoluteIDQ Stero17 kit can analyse up to 80 samples per batch, requires a sample volume of only 500 μL, and enables the most comprehensive simultaneous quantification of a steroid panel using HPLC-MS/ MS as well as improved high-throughput UHPLC–MS/MS analysis. The kit covers the standardized quantitative analysis of 17 of the most important steroid hormones from only a single blood sample using UHPLC-MS/MS, a technology meeting the most stringent quality control criteria for routine diagnostics, ensuring a high level of analytical sensitivity and selectivity, and guaranteeing superior accuracy and excellent precision for robust results. The kit, which has been approved by proficiency tests, comes complete with isotopelabelled internal standards, reagents and protocols for sample pre¬paration based on solid-phase extraction in a 96-well plate format and subsequent UHPLC-ESI-MS/ MS analysis in highly specific multiple reaction monitoring (MRM) mode and a tightly quality-controlled workflow. Given the significant role of steroid hormones in the body, their quantitative analysis in clinical diagnostics and research-based applications can be crucial. Compared to immunoassay-based tests, LC-MS/MS analysis offers the advantages of multiplexing (simultaneous analysis of multiple target analytes), higher specificity (by so-called multiple reaction monitoring (MRM), greater dynamic range of the calibration and lower inter-laboratory variability (including diversity in sample preparation, calibrators and analytical methods).
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The Full Picture in Urinalysis
, /in Product News /by 3wmediaThe second generation of the popular automated urinalysis system that combines a urine chemistry analyzer and a urine sediment analyzer is introduced. The LabUMat 2 is designed for high volume urine test strip reading and for analyzing three physical parameters. The UriSed 2 is able to detect urine particles in urine sediment with high accuracy. Relying on the automation of traditional manual microscopy and advanced image processing the sediment analyzer offers a unique method for sedimentation. It performs sample preparation, provides whole field of view microscopic images of sediment and identifies urine particles in a fully automated way, while human evaluation is also possible any time after the measurement on screen, without manual microscopy. The interfaced LabUMat 2 & UriSed 2 analyzers form a Complete Urine Laboratory System working without any special liquid reagents. This latest development of 77 Elektronika is a walk-away system dealing with the workload of big laboratories. The automated technology improves diagnostic quality by increased reproducibility and accuracy and also improves productivity by eliminating the time consuming procedure of manual sample preparation.
More info – contact: sales@e77.hu
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Elisa assay for West Nile virus
, /in Product News /by 3wmediaWest Nile virus (WNV) is becoming widespread: it has been detected in over half of the 50 states of the USA and exposure to it causes a disease with a number of symptoms including encephalitis. The West Nile Detect IgG Test aids the diagnosis of human exposure to the West Nile virus by an ELISA assay system for the detection of antibodies in human serum to WNV derived recombinant antigen (WNRA). WNRA, which can be used as a rapid serological marker for WNV infection, is a recombinant antigen consisting of a stretch of peptides from two WNV antigens. This assay – a two-step capture ELISA – uses a small 4 μL sample volume, has a specificity of 98.4% and a sensitivity of 96.2% and each kit contains reagents for 96 tests. The results are determined by absorbance measurement at 450 nm. Above a certain threshold, the ratio of the absorbances of the WNRA and the control wells accurately determines whether antibodies to WNV are present and a set of positive and negative samples is provided as internal controls in order to monitor the integrity of the kit components. Stored at 2–8 °C, the kit has a shelf life of 12 months.
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Human apoliprotein H Elisa
, /in Product News /by 3wmediaApolipoprotein H (ApoH) is a major antigen for antiphospholipid antibodies present in patients with antiphospholipid syndrome (APS), which is related to venous and arterial thrombosis, fetal loss, and thrombocytopenia. Reports show that ApoH may function in blood coagulation and in the clearance of apoptotic bodies from circulation. Serum concentrations of ApoH have been found to be abnormal in patients with primary hyperlipidemia. Serum ApoH concentrations are increased in type 2 diabetes patients with microalbuminuria, suggesting a relationship with microvascular complications. ApoH was described as a major urinary protein in the urine of patients with renal tubular disorders such as Wilson´s disease, Dent´s syndrome, Fanconi´s syndrome and renal tubular acidosis. The Human ApoH ELISA kit extends BioVendor’s portfolio of diagnostic kits for lipoprotein metabolism, renal and cardiovascular disease and diabetology. Suitable for serum, plasma (EDTA, citrate, heparin) and urine samples, the 96 well-microtitre plate format is suitable for use with standard ELISA equipment.
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ELISA assay for 25-Hydroxyvitamin D
, /in Product News /by 3wmediaBased on recently developed monoclonal antibodies, an innovative easy-to-use ELISA assay with all-in-one (AIO) technology for the measurement of 25-Hydroxyvitamin D TOTAL (D2 and D3) has been launched. These antibodies have already been successfully used in the 25-Hydroxyvitamin D TOTAL RIA assay. Specifically designed to be used manually as well as on open automated systems, this ELISA uses a pre-treatment step that is executed directly in the assay well, and has a total assay time of less than 4 hours. The assay has been validated on the Stratec GEMINI open ELISA automated analyser (Stratec Biomedical GMBH, Germany) and a validated protocol is available to all Stratec GEMINI users. Offering major advantages with respect to ease-of-use to laboratory technicians (AIO technology), this assay is ideal for medium-sized and smaller labs with a throuput of 10 to 80 samples per day. Higher sample numbers can easily be run on any open automated ELISA platform. Assays for 25-Hydroxyvitamin D3 specific RIA , 25-Hydroxyvitamin D Total RIA and 1,25-Dihydroxyvitamin D RIA, as well as monoclonal antibodies against 25 Hydroxyvitamin D (total), are available for use by other assay developers.
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Nitrocellulose membranes optimized for diagnostic assay reproducibility
, /in Product News /by 3wmediaDesigned for diagnostic assays, the Whatman FF High Performance (HP) membrane enables fast, razor-sharp line separation and highly reproducible results for the detection of target molecules in liquids such as water, urine, blood and saliva. Th e target molecules may include drugs, hormones, proteins, antibodies, nucleic acids, whole bacteria and viruses. Th e membrane is suitable for use in lateral fl ow assay manufacture as it provides improved reliability and rapid results. Production using an innovative casting method yields a uniform, new powderfree surface that delivers a coeffi cient of variation in capillary rise of < 10 %. Both the intra- and inter- lot consistency was tested through analysis of over 1000 samples giving a low coeffi cient of variation. Th is excellent reproducibility improves the consistency of assays and off ers a more reliable limit of detection, which in turn reduces the costs of assay optimization for researchers. Th ree FF HP membranes are available with diff erent capillary rise times (wicking rate), allowing researchers to choose the most suitable membrane for their assay. Read more
Component-resolved allergy diagnostics
, /in Product News /by 3wmediaSensitizations to birch and grass pollens – two of the most common inhalation allergies – can be characterized to a new level of detail using a multiparameter, component-resolved immunoblot test system from EUROIMMUN. The assay employs single purified allergen components (SPAC) for the detection of specific IgE antibodies in place of the usual whole extracts in an approach known as molecular allergology. Th is in-depth profiling enables allergologists to precisely identify diseasecausing allergens, assess the risk of cross allergies and determine patients’ suitability for specific immunotherapy. The immunoblot contains all major allergy-inducing proteins from birch pollen (Bet v1, Bet v2, Bet v4, Bet v6) and grass pollen (Phl p1, Phl p5, Phl p7, Phl p12). In a published study the test successfully verified birch or grass pollen sensitizations in 77 patients with clinically confirmed allergies, and correlated well with comparable commercial assays. Th e assay is based on established EUROLINE technology, which combines economy and efficiency in allergy testing. Only small amounts of sample material are required, and the procedure can be fully automated using innovative devices (EUROBlotMaster, EUROBlotScanner, EUROBlotCamera) and soft ware (EUROLineScan). Th e new EUROLINE SPAC Pollen 1 Profile supplements the EUROLINE portfolio, which offers an extensive range of application-oriented profiles at a very competitive price per allergen.
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