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Author Archive for: 3wmedia
Entries by 3wmedia
The whooping cough epidemic: is the ‘new’ vaccine to blame?
Pertussis (whooping cough) has been a significant cause of morbidity and mortality in young children since the first epidemic was described in 1578. Currently in the West even when infants suffering from the disease are hospitalized and appropriately treated, around 1% still die, and in less developed countries the mortality rate in infants is as […]
Diagnosis of blood-borne parasitic infections: an overview
Methods for the diagnosis of blood-borne parasitic infections have stagnated in the last 20–30 years. However, recently, there has been a tremendous effort to focus research on the development of newer diagnostic methods focusing on serological, molecular, and proteomic approaches. This article examines the various diagnostic tools that are being used in clinical laboratories, optimized […]
Molecular diagnosis and sub-speciation of cutaneous leishmaniasis
Diagnosing cutaneous leishmaniasis histologically depends on the identification of the amastigotes, which is inconclusive and leads to cases of missed diagnosis or misdiagnosis. In this article, we describe a rapid diagnostic molecular method for Leishmania species identification and differentiation using DNA extracted from formalin-fixed paraffin-embedded (FFPE) skin tissue biopsies. by L. Yehia and Dr I. […]
Real time RT-PCR is the gold standard for laboratory diagnosis
Noroviruses are the most common cause of viral gastroenteritis in humans. In recent years diagnostic methods for Noroviruses, especially real-time reverse transcription-polymerase chain reaction (RT-PCR) for the detection of Norovirus-RNA, have been improved and become more widely available. by Dr Christoph Metzger-Boddien Noroviruses are transmitted by fecally contaminated food or water, by person-to-person contact, and […]
Quality control: the emergence of risk-based analysis
One of the fastest-paced developments in clinical laboratories has been in the area of quality control (QC) systems. Its driver has been the increase in the performance and sophistication of QC software, which has progressively tightened benchmarks for acceptable standards. On the plus side, improved QC systems clearly help a laboratory to serve the needs […]
New technology allows previously esoteric testing to be performed in a core laboratory
FilmArray is a highly automated small instrument capable of detecting infectious agents using PCR technology. Due to its simplicity the tests could be performed in a rapid-response core laboratory by general medical technologists. This operational model has demonstrated achievements in reducing turn-around-time and thus improved patient care. by Dr M. Xu, Dr X. Qin, Dr […]
ESBL NDP and Carba NP tests: novel techniques for rapid detection of multidrug-resistant bacteria
Two novel biochemical tests, the ESBL NDP and the Carba NP tests, have been recently developed for the early detection of ESBL- or carbapenemase resistance traits in Enterobacteriaceae. Those tests are rapid, sensitive, specific and cost-effective. Implementation of those tests in clinical microbiology laboratories may significantly improve the management and outcome of patients. by Dr […]
Shedding light on obesity and vitamin D status
The worldwide prevalence of obesity (defined as a BMI greater than 30) has more than doubled in the last thirty years, largely as a result of lifestyle changes leading to many people having a greater energy intake than expenditure. According to a study published in The Lancet recently, with the exception of populations in sub-Saharan […]
Recent trends in malignant melanoma biomarker research
Melanoma is the most malignant type of all skin neoplasms. Although current clinical, morphologic, pathologic, and biochemical methods provide insights into disease behaviour and outcome, melanoma is still an unpredictable disease. Once in an advanced stage, it remains a fatal neoplasm with few therapeutic options. Therefore, significant efforts still need to be made in finding […]