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Author Archive for: 3wmedia
Entries by 3wmedia
Does Zika virus (ZIKV) cause fetal microcephaly?
In February the WHO declared that the spread of ZIKV infection, which is sweeping through South and Central America resulting in more than a million cases so far, was a global health emergency. Infection with this flavivirus, endemic for decades in areas of Africa and Asia where the vector Aedes species mosquitoes breed, is usually […]
Mass spectrometry detection of bacterial toxins and antibiotic resistance
Mass spectrometry (MS) and proteomics are gaining popularity in bacterial research and applications; notably, bacterial toxin detection and antibiotic resistance. Currently, several MS platforms exist (Fig. 1) and are described below. by Angela Sloan, Dr Keding Cheng Mass pattern and spectra comparison using MALDI-TOF-MSThe United States Food and Drug Administration (US FDA)-approved MALDI Biotyper or […]
Improving the diagnosis of peri-prosthetic joint infections
Prosthetic joint infections are a devastating complication of arthroplasty. Despite this, current culture techniques lack sensitivity and not standardized. We examined the inoculation of peri-prosthetic tissue specimens into blood culture bottles, demonstrating improved sensitivity compared to conventional methods. These findings influence patient care, allowing accurate diagnosis of infection. by Dr T. N. Peel, B. L. […]
Automating the clinical microbiology lab
In spite of some exceptions, the clinical microbiology lab has been a late starter as far as automation is concerned. It has also traditionally been viewed as ‘low tech’, especially when compared to its cousins in clinical chemistry or pathology. A variety of factors, however, have been converging to reverse such a situation. Automation hampered […]
Trends in infectious diseases and clinical microbiology at ECCMID 2016
Keynote sessions on antimicrobial resistance, the microbiome and systems vaccinology as well as presentations on late-breaking research on refugee health and colistin resistance at ECCMID 2016 The annual meeting of the European Society of Clinical Microbiology and Infectious Diseases is taking place this year from April 9 – 12 in Amsterdam. At the world’s largest […]
Amplification-free direct detection of Ebola virus on a hybrid optofluidic platform
Low-complexity detection of infectious diseases with high sensitivity and specificity is urgently needed, especially in resource-limited settings. Optofluidic integration combines clinical sample preparation with optical sensing on a single chip-scale system, enabling the direct, amplification-free detection of single RNA from Ebola viruses. The optofluidic system fulfils all key requirements for chip-based clinical analysis, including a […]
Visual detection of Ebola virus: targeting the NP gene by RT-LAMP
Ebola virus (EBOV) can lead to severe hemorrhagic fever with a high risk of death in humans and other primates. More recently, reverse transcription loop-mediated isothermal amplification (RT-LAMP) has become readily available for the diagnosis of EBOV, and is a suitable tool for clinical screening, diagnosis and primary quarantine purposes. by H. Li, W. Lin, […]
Biochemical markers of alcohol intake
Biochemical markers of alcohol intake can be separated into two categories: direct markers of ethanol metabolism and indirect markers. The different alcohol markers have varying time windows of detection and are a useful additional tool to detect alcohol intake in alcohol-dependent clients. by Jane Armer and Rebecca Allcock IntroductionAlcohol dependence is characterized by craving, tolerance, […]
Meeting clinical needs with high performance viral load assays, workflow improvements and reduced turnaround times
Niguarda Hospital in Milan is one of Italy’s leading general hospitals, and provides an extensive range of medical disciplines for adults and children throughout the Lombardy region and beyond. Our hospital’s Department of Laboratory Medicine aim is to offer a complete, continuous and prompt diagnostic laboratory testing service, in order to guarantee effective support for […]